Neuroscience 2005 Abstract
| Presentation Number: | 316.14 |
|---|---|
| Abstract Title: | Gene-expression profiling of auditory fear extinction in the rat medial prefrontal cortex. |
| Authors: |
Vazquez, A.*1
; Sepulveda, M.2
; Wang, J.1
; Saavedra, L.2
; Ortiz-Zuazaga, H.3
; Quirk, G.2
; Pena de Ortiz, S.1
1Biology, Univ. Puerto Rico, San Juan, Puerto Rico 2Ponce School of Medicine, Univ. Puerto Rico, San Juan, Puerto Rico 3Computer Science, Univ. Puerto Rico, San Juan, Puerto Rico |
| Primary Theme and Topics |
Cognition and Behavior - Animal Cognition and Behavior -- Emotional learning, memory systems, and modulation of memory |
| Secondary Theme and Topics | Cognition and Behavior<br />- Motivation and Emotion<br />-- Emotions |
| Session: |
316. Emotional Learning II Poster |
| Presentation Time: | Sunday, November 13, 2005 2:00 PM-3:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # QQ9 |
| Keywords: | extinction, NMDA, microarrays, mPFc |
Previous studies have shown that long-term retention of fear extinction requires the medial prefrontal cortex (mPFC). Inhibition of NMDA receptors (Burgos-Robles et al., SFN, 2004) or protein synthesis (Santini et al., 2004) in the mPFC impairs consolidation of fear extinction. Here we used Agilent 22K rat oligonucleotide microarrays to identify genes that are regulated within the mPFC during extinction. Four experimental groups were studied: 1) Rats receiving tone fear conditioning on Day 1 and extinction training on Day 2 (EXT); 2) Same as above but with a systemic injection of the NMDA antagonist CPP (10 mg/kg) prior to extinction (EXT-CPP); 3) Rats that received acquisition but no-extinction (No-EXT); 4) Rats given a full set of tones but never shocked. Extinction was given in a different context than conditioning, and rats were sacrificed 30 min after extinction training. Total RNA was obtained from the mPFC, linearly amplified, and labeled using fluorescent dyes Cy3 or Cy5. Data from two biological replicates have been analyzed. Of 22,000 genes assayed, 59 showed extinction-specific upregulation, and included protein kinase C gamma, arginine vassopresin, and VGCC gamma 8 subunit. Only 7% of extinction-specific genes depended on NMDA receptor activation, and included synaptic vesicle glycoprotein 2a (Sv2a). Gene expression profiling may help us begin to understand the molecular basis of tone fear extinction in the mPFC.
Supported by NIH (SPO grant & NIGMS S06GM08102, NCRR 5P20 RR15565-02); GJQ RO1 MH058883 & SO6 GM08239
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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