Neuroscience 2001 Abstract
| Presentation Number: | 332.18 |
|---|---|
| Abstract Title: | Sodium magnetic resonance imaging (MRI) for Monitoring the Increase in Brain Tissue Sodium Concentration, [Nа<sup>+</sup>], in Rat Focal Cerebral Ischemia Identified with MAP2. |
| Authors: |
Jones, S. C.*1
; Kharlamov, A.1
; Kim, D. K.2
; Reid, E. D.3
; Boada, F. E.3
1Anesthesiology, Allegheny General Hospital, Pittsburgh, PA 2Neurosurgery, Allegheny General Hospital, Pittsburgh, PA 3MR Res Ctr, Univ Pitt Med Ctr, Pittsburgh, PA |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Ischemia -- Neuroprotection and tolerance |
| Secondary Theme and Topics | Autonomic, Limbic and Other Systems<br />- Brain Metabolism, Transport and Blood Flow |
| Session: |
332. Ischemia: neuroprotection and tolerance V Poster |
| Presentation Time: | Monday, November 12, 2001 9:00 AM-10:00 AM |
| Location: | Exhibit Hall ZZ-55 |
| Keywords: | MCAO, BRAIN IMAGING, IMMUNOHISTOCHEMISTRY, STROKE |
Brain [Na+] has been proposed to assess insult duration in evolving stroke. Advances in sodium MRI make possible the measurement of the rate of [Na+] increase in a rat model of cortical ischemia. In five Sprague-Dawley rats (~400 g) focal cortical ischemia was induced with occlusion of the distal left middle cerebral artery and both common carotid arteries under isoflurane anesthesia. Sodium 3D data were acquired on a 3 T whole body scanner (GE MS), using a dual-tuned (23Na/1H), dual-quadrature birdcage RF coil. [Na+] maps (twisted projection) were acquired every 5.3 min from which the [Na+] in the ischemic and normal cortex was measured. The infarct was located using T2-weighted 1H images and immunoreactivity to MAP2 (HM2 ab, Sigma). The figure shows the time course of the [Na+] from the ischemic and normal cortex for one animal. [Na+] did not increase in normal cortex (0.02 ± 0.03 %/hr). In ischemic cortex, [Na+] increased at mean rate of 16 ± 3 %/hr (p < 0.001), comparing favorably with 23 ± 4 %/hr (n = 16) using direct tissue analysis (Wang et al, Stroke 31:1386, 2000). Using the MR method, the entire [Na+] time course can be obtained in one animal.
Supported by NIH NS30839, HL64205 & Whitaker Foundation
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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