Neuroscience 2002 Abstract
| Presentation Number: | 341.17 |
|---|---|
| Abstract Title: | Relative distribution of mutually exclusive splice variants of exon 37 of human Ca<sub>v</sub>2.1 calcium channels in different regions of the brain. |
| Authors: |
Chang, S. Y.*1
; Yue, D. T.2
; Soong, T. W.1,2,3
1 , Nat' Neurosci Inst, Singapore, Singapore 2Dept of Biomed Engr, Johns Hopkins Univ Sch Med, Baltimore, MD 3Dept of Physiol, Nat' Univ of Singapore, Singapore, Singapore |
| Primary Theme and Topics |
Synaptic Transmission and Excitability - Ion Channels -- Calcium channels - Modulation |
| Session: |
341. Ion channels: Ca++ channels III Poster |
| Presentation Time: | Monday, November 4, 2002 1:00 PM-2:00 PM |
| Location: | Hall A2-B3 C-47 |
| Keywords: | EF hand |
The carboxyl-terminus of Cav2.1 P/Q-type calcium channel plays an important role in Ca2+-dependent facilitation and inactivation (Lee et al, Nature 399:155; DeMaria et al, Nature 411:484). The EF hand lies within this region and is encoded by exons 36 and 37. In Cav2.1 calcium channels, exon 37 exhibits mutually exclusive splice variation, giving rise to either exon 37a or 37b. Facilitation is observed when exon 37a is expressed but not exon 37b (Chaudhuri et al, SFN abstract, 2001). We report here the relative distribution of the two splice forms across different regions of the human brain. Eight human cDNA libraries were analyzed: fetal whole brain, adult whole brain, amygdala, cerebellum, cerebral cortex, hippocampus, substantia nigra and thalamus. For each library, the PCR amplicons spanning exons 35 to 38 were cloned and more than 200 bacterial colonies were screened using exon 36 and exon 37a- or 37b-specific primers. The results indicated that exon 37a is predominantly expressed in the substantia nigra and thalamus, while exon 37b is predominantly expressed in the amygdala and fetal whole brain. The two splice variants are equally expressed in adult whole brain, cerebellum, cerebral cortex and hippocampus. There is a developmental switch from a predominant expression of exon 37b in fetal brain to an equal expression of both variants in adult brain. The uneven distribution of the two splice forms in different brain regions adds to the rich repertoire of Cav2.1 channel behavior in neuronal activities.
Supported by NMRC, Singapore
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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