Neuroscience 2002 Abstract
| Presentation Number: | 329.8 |
|---|---|
| Abstract Title: | Growth and Differentiation of mesencephalic Neural progenitors: Effect of serum and extra cellular matrix. |
| Authors: |
Takeshima, T.*1
; Fukuhara, Y.1
; Kashiwaya, Y.1,2
; Nakaso, K.1
; Araga, S.1
; Nakashima, K.1
1Neurology, Tottori Univ Fac Med, Yonago, Japan 2LMBB, NIAAA-NIH, Rockville, MD |
| Primary Theme and Topics |
Development - Neurogenesis and Gliogenesis -- Neural Stem Cells |
| Secondary Theme and Topics | Development<br />- Neurogenesis and Gliogenesis<br />-- Neuronal differentiation |
| Session: |
329. Neurogenesis and gliogenesis: neural stem cells--signaling Poster |
| Presentation Time: | Monday, November 4, 2002 4:00 PM-5:00 PM |
| Location: | Hall A2-B3 A-1 |
| Keywords: | dopamine, Parkinson's disease, neural stem cell |
We have reported a mesencephalic microisland culture method (J Neurosci 1994; 14: 4769-79) and expansion of dopaminergic neurons with microisland culture (SFN Abstr. 1997;23:593). We explored further expansion of mesencephalic neural progenitors (MNP) and their differentiation. MNPs were prepared from 1mm3 tissue blocks isolating from E14 rat ventral mesenscephalon. Dispersed cells were cultured in 75cm2 flasks (5.0 x 105 cells/flask) using an expansion medium (EM; DMEM/F12+TIPS+ bFGF) with/without serum priming (SP; 24hours-2% FCS). Expanded MNPs were collected at the 14 days in cultures and were frozen for later experiments. Striatal neural progenitors (SNPs) were also prepared by the same procedures. Thawed MNPs and SNPs were cultured 7 days in EM with/without SP. Growth rates of MNPs were 33 folds in SP cultures and 123 folds in serum-free cultures. On the contrary, those of SNPs were 111 folds and 13.2 folds, respectively. These results indicated that the effects of serum to neural progenitors are different between sources of the progenitors. At the passages, 50% of MNPs were MAP2+ and 20% were Vimentin+. Expanded MNPs were plated on 8-well chamber slides coated with various substrates, i.e. poly-D-lysine (PDL), fibronection, collagen and laminin. At 5 days, cultures were evaluated by double immunostaining with MAP2 and TH. The strength order of differentiation was fibronectin> PDL> laminin ≧ collagen ≒ uncoated glass. This culture technique will provide new experimental model of dopaminergic neurons relevant to Parkinson's disease.
Supported by Min. Edu., Culture, Sports, Sci & Technology, Gov.Japan
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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