Neuroscience 2005 Abstract
| Presentation Number: | 268.9 |
|---|---|
| Abstract Title: | Chronic stress reduces serotonin transporter function and potentiates the behavioral effects ethanol. |
| Authors: |
Baganz Strode, N. L.*1
; Boyce-Rustay, J. M.
; Munn, J. L.1
; Holmes, A.
; Daws, L. C.1
1Physiology, Univ. of Texas HSC, San Antonio, TX |
| Primary Theme and Topics |
Neural Excitability, Synapses, and Glia: Cellular Mechanisms - Transporters -- Monoamine |
| Session: |
268. Monoamine Transporter III Poster |
| Presentation Time: | Sunday, November 13, 2005 1:00 PM-2:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # F34 |
| Keywords: | HIPPOCAMPUS, SEDATIVE/HYPNOTIC, CHRONOAMPEROMETRY, CLEARANCE |
Stress has profound effects on serotonin (5-HT) neurotransmission. The 5-HT transporter (5-HTT) provides the primary mechanism for termination of 5-HT neurotransmission and there is evidence to suggest it is under regulatory control of hormones released in response to stress. Stress is also linked to alcoholism. We recently reported (SfN 2004 Prog# 54.15) that the behavioral and neural effects of ethanol are potentiated in mice lacking the 5-HTT, further underlining the importance of 5-HTT function in determining drug response. Here we extend our investigation to examine the relationship between chronic stress, 5-HTT function and sensitivity to ethanol in vivo. Male C57BL/6J mice were exposed to chronic stress (daily 10 min forced swim for 14 days) or were unhandled (control). 24 hrs following the final stress session, mice were either anesthetized and prepared for chronoamperometry to measure 5-HT clearance in CA3 region of hippocampus, or tested in behavioral assays. Compared to control mice, 5-HT clearance was drastically reduced (2-fold) in stressed mice. However, in contrast to 5-HTT KO mice, where the effect of ethanol to inhibit 5-HT clearance was greatly potentiated, ethanol inhibited 5-HT clearance to a similar extent in stressed and non-stressed mice. Behaviorally, the response of stressed mice to ethanol mirrored our observations in 5-HTT KO mice: the response of stressed C57BL/6J mice to both the sedative/hypnotic and hypothermic effects of ethanol were potentiated relative to controls. Altered metabolism of ethanol could not account for these differences. These data suggest that reduced 5-HTT function, as a consequence of either genetic or environmental manipulation, produces enhanced behavioral responses to ethanol. In contrast, adaptive changes that occur in response to a genetic reduction in 5-HTT expression appear requisite for the enhanced ability of ethanol to inhibit 5-HT clearance.
Supported by RO1-MH64489 & NARSAD (LCD) and NIAAA-IRP (JMB-R, AH)
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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