Neuroscience 2000 Abstract
| Presentation Number: | 281.15 |
|---|---|
| Abstract Title: | Characterization of the early and late phase of stroke in the penumbra zone and regions of degeneration. |
| Authors: |
Andersson, B.*1
; Abo, M.1
; Chen, Z.1
; Lai, L. J.1
; Bjelke, K.1
; Bjelke, B.1
1MR-Center, Karolinska Institute, Stockholm , Sweden |
| Primary Theme and Topics |
J. Disorders of the Nervous System and Aging - 133. Ischemia: cellular and molecular mechanisms |
| Secondary Theme and Topics | H. Other Systems of the CNS<br />- 101. Brain metabolism and blood flow |
| Session: |
281. Ischemia: cellular and molecular mechanisms IV Poster |
| Presentation Time: | Monday, November 6, 2000 10:00 AM-11:00 AM |
| Location: | Hall G-J |
| Keywords: | Motor cortex, Jscheulia, UHIN Structure, Apoptosis |
Introduction: By using two experimental rat models, resulting in neuronal injury in the brain, we can mimic the early and late phase of stroke. We want to characterize the death pathways and the two different types of cell death, apoptosis and necrosis, in the damaged brain tissue. We evaluate the lesions by the nerve cells morphologically, using electron microscopic (EM) techniques in combination with behavioural tests and magnetic resonance imaging (MRI). Methods: Male Spraque-Dawley rats are used. The model of compression lesion results in a penumbra zone where mainly apoptosis is seen, while the late phase stroke model results in pan-necrotic tissue. At the end of the experiment the rats are transcardially perfused, using 3% glutaraldehyde and then prepared for EM examination. After fixation the tissue is dehydrated and embedded in Agar 100, a resin of epoxy type. Ultra thin sections, mounted on formvar coated copper grids, are stained with uranyl acetate and lead citrate, The sections are examined in a transmission electron microscope. Results: At this ultrastructural level we can observe morphological changes in the neurons, such as chromatin margination in the nucleus, DNA fragmentation and membrane rupture and blebbing. These changes can be assigned to either apoptotic or necrotic cell death in different stages. The lesioned brain tissue shows the expected characteristics of cell death for the two respective stroke models. Also in the unlesioned tissue we the found morphological changes that indicate cell death. Conclusion: Combining the three evaluation platforms of the stroke models, we have a possibility to define the therapeutic windows where we can add drugs, thus improving the behavioural outcome for stroke patients. Acknowledgements: Trygg Hansas Forskningsstiftelse, David och Astrid Hageléns stiftelse, Stroke-Förbundets Stiftelser och Fonder, Knut och Alice Wallenbergs stiftelse.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2000 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2000. Online.
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