Neuroscience 2005 Abstract
| Presentation Number: | 250.12 |
|---|---|
| Abstract Title: | Activity dependence of long-term slice cultures on cerebellar granule cell survival. |
| Authors: |
Hillman, D. E.*1,2
; Chen, S.1,2
; Bing, R.2
; Sugimori, M.2
; Llinas, R.2
1Otolaryngology, New York Univ. School of Medicine, New York, NY 2Physiology & Neuroscience, New York Univ. School of Medicine, New York, NY |
| Primary Theme and Topics |
Neural Excitability, Synapses, and Glia: Cellular Mechanisms - Glia-neuron interactions -- Other |
| Secondary Theme and Topics | Development<br />- Developmental Cell Death<br />-- Developmental cell death: Biological effects |
| Session: |
250. Developmental Cell Death: Biological Effects I Poster |
| Presentation Time: | Sunday, November 13, 2005 4:00 PM-5:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # B21 |
| Keywords: | slice cultures, granule cells, Cell death |
The majority of granule cells in lobule 1-8 of 12 day old, sagittally-sliced rat cerebella, die or fail to develop in the first 7 days of standard media cultures while Purkinje cells remain in large number. Previously, we demonstrated axonal growth and robust synaptogenesis at 14 DIV when the [K+]o was raised to 20 to 40 mM after 7 days of initial placement in standard [K+]o (Chen et al., SFN Abst 2003). Dissociated cell cultures of rat granule cells survived better in 25 mM [K+]o (Hacket al., 1993 ) and released transmitter (Cousins et al., 1997; Varming et al., 1997). In the present study, we asked if there are levels of [K+]o that further enhances circuit development in slice cultures maintained for extended periods. Slices were treated with 5, 10, 15 or 20 mM [K+]o either from the time of slicing to 14 days or after an initial 7 day period in 5 mM [K+]o. We were surprised to find that slices cultured, immediately, in 15 mM [K+]o had uniquely optimal granule layer survival in most lobules. This finding contrasted sharply with corresponding slices in 5 or 10 mM culture media where there was only a narrow stained layer of granule cells within lobules 1-8. These findings together with previous ones suggest a critical level of [K+]o that promotes activity dependent survival of granule cells as well as axonal growth, synaptic vesicle production and synaptogenesis.
Supported by NIH-NINCDS NS-13742 & NYSCIR
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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