Neuroscience 2004 Abstract
| Presentation Number: | 216.13 |
|---|---|
| Abstract Title: | Curcumin (Curc) or its metabolite tetrahydrocurcumin (THC) reduces the production of nitric oxide synthase (iNOS) in mouse brain. |
| Authors: |
Begum, A. N.*1
; Heath, D. D.3
; Lim, G. P.1
; Morihara, T.1
; Kim, P. H.1
; Rock, C. L.3
; Cole, G. M.1,2
; Frautschy, S. A.1,2
1Med., UCLA and Greater Los Angeles Healthcare Syst., GRECC, Sepulveda, CA 2Neurol., UCLA and Greater Los Angeles Healthcare Syst., GRECC, Sepulveda, CA 3CA, UC, 91343, |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Neurodegenerative Disorders -- Alzheimer's Disease: Therapies |
| Secondary Theme and Topics | Neurological and Psychiatric Conditions<br />- Neurodegenerative Disorders<br />-- Alzheimer's Disease: Neuropharmacology and neurotransmitters |
| Session: |
216. Neuroprotective Treatments and Mechanisms I Poster |
| Presentation Time: | Sunday, October 24, 2004 8:00 AM-9:00 AM |
| Location: | San Diego Convention Center - Hall A-H, Board # RR12 |
| Keywords: | AD therapies |
Curcumin (Curc) and tetrahydrocurcumin (THC) are both phenolic compounds, exerting antioxidant and anti-inflammatory properties that contribute to neuroprotection, for example, in Alzheimer’s Disease (AD) and in chemoprevention. One inflammatory pathway that has been implicated in both diseases is iNOS which catalyzes the production of nitric oxide (NO). Here we examine the bioavailability and anti-inflammatory effects of Curc and THC with different routes of administration: (gavage, GV; intra-muscular, IM; or intra-peritoneal, IP). Mice were injected IP with LPS (lipopolysaccharide, 0.5 µg/g body weight) or vehicle and iNOS production measured in mouse brain. We administered Curc and THC by GV, IP and IM (0.4, 0.4,and 0.2µmoles, respectively) to C57bl mice with or without LPS and collected their plasma and brain. IM-injected Curc and THC resulted in their elevation in plasma and brain by HPLC detection,and significantly reduced CNS iNOS production by Western. Two-step QPCR studies of mouse brain tissue also showed IM Curc and THC decreased LPS induction of iNOS mRNA expression by 85% and 80% respectively. Higher (1.3 µmole) GV doses of Curc were required to detect in plasma by HPLC but low doses of Curc or THC by GV or IP still significantly (P<0.05) reduced iNOS mRNA. In conclusion, low dose IM administration of Curc or THC can be detected in plasma and brain, but low oral doses also reduced iNOS protein and mRNA. This would support a role for Curc or its major metabolite THC in the anti-inflammatory effects observed in Alzheimer models.
Supported by NIA-AGT10685(SAF)
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
Copyright © 2004-2025 Society for Neuroscience; all rights reserved. Permission to republish any abstract or part of any abstract in any form must be obtained in writing by SfN office prior to publication.
