Neuroscience 2001 Abstract
| Presentation Number: | 245.3 |
|---|---|
| Abstract Title: | MRI guidance improves accuracy of stereotactic targeting of cell transplantation and gene therapy in parkinsonian monkeys. |
| Authors: |
Subramanian, T.*1
; Venkiteswaran, K.1
; Marchionini, D.3
; Brummer, M.2
1Cleveland Clinic Foundation, Cleveland, OH 2Emory Univ., Atlanta, GA 3Rush-Presbyterian Univ., Chicago, IL |
| Primary Theme and Topics |
Development - Transplantation and Regeneration -- Transplantation |
| Session: |
245. Transplantation and regeneration: transplantation I Slide |
| Presentation Time: | Monday, November 12, 2001 8:30 AM-8:45 AM |
| Location: | Room 28D |
| Keywords: | nonhuman primates, Parkinson's disease, neurotransplantation, neurodegeneration |
Accuracy of targeting is critical for the success of cell transplantation and recombinant viral vector mediated gene therapy. We compared the accuracy of conventional atlas guided stereotaxis to MRI guided stereotactic targeting into various basal ganglia nuclei in hemiparkinsonian (HP) monkeys. High resolution 3-D T1 weighted MR images of the brain were obtained in 27 HP monkeys fitted with a MRI compatible stereotactic frame. This was immediately followed by cranial surgery with the frame in situ. Thirteen additional HP monkeys underwent stereotactic atlas guided cranial surgery for placement of cell transplants. Following extensive behavioral testing, microelectrode recording (MER) and PET imaging all animals were euthanized and perfused with 4% paraformaldehyde. The brains were cut in coronal sections and stained to determine the accuracy of stereotactic placements. Preliminary data analysis indicate that MRI guided stereotaxis was accurate (2 caudate and 3 putamen targets) in 16 out of 16 animals analyzed so far. Atlas guided stereotaxis was accurate in 9 out of 13 animals. Targeting error occurred most in the posterior putamen. These results suggest that accuracy of stereotactic cranial targeting into the putamen and the caudate in HP monkeys can be improved with high resolution 3D MR imaging and immediate surgery with the head frame in situ. Data from 6 additional monkeys indicate that high resolution MRI guidance combined with neuronal MERs has the highest accuracy for stereotactic targeting into smaller nuclei like the subthalamic nucleus and the substantia nigra.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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