Neuroscience 2004 Abstract
| Presentation Number: | 167.4 |
|---|---|
| Abstract Title: | Gender differences in reserpine-induced dopamine output in nigrostriatal system of mice. |
| Authors: |
Bhatt, S.*1
; Dluzen, D.1
1Dept. of Anat., NEOUCOM, Rootstown, OH |
| Primary Theme and Topics |
Synaptic Transmission and Excitability - Transporters -- Monoamines, GABA, etc. |
| Secondary Theme and Topics | Motor Systems<br />- Basal Ganglia<br />-- Physiology of transmitters and receptors |
| Session: |
167. GABA/Dopamine Transporters Poster |
| Presentation Time: | Sunday, October 24, 2004 11:00 AM-12:00 PM |
| Location: | San Diego Convention Center - Hall A-H, Board # K20 |
| Keywords: | PARKINSON, SEX DIFFERENCES, UPTAKE, STRIATUM |
Most reports of Parkinson’s disease indicate that males have a higher prevalence. This can be studied within the laboratory as sex differences are present within animal models of Parkinson’s disease. We have reported that female mice show a higher striatal Dopamine (DA) uptake and decreased 3,4-dihydroxyphenylacetic acid (DOPAC) production as compared to male mice suggesting a gender difference in vesicular functioning. (SFN 2003 Poster #706.10)
To further analyze these gender differences in the vesicular transporter mechanisms, we infused different concentrations of Reserpine (10µM, 20µM or 50µM) during superfusion of male (n=4) and female (n=4) corpus striatum tissue. After a three-interval basal collection period, the Reserpine solution was infused for a two collection interval period. The normal superfusion medium was resumed with the start of collection interval five and continued until the termination of the superfusion at collection interval eight.
The amount of DA obtained in effluent samples following Reserpine infusion showed a dose-dependent increase in males and were significantly greater compared with that from females at the 20µM and 50µM dose.
Mean ± SEM DA in pg/mg/min in Males vs Females:
10µM – 7.9 ± 4.3 vs 9.5 ± 2.5,
20µM – 20.4 ± 11.4 vs 2.9 ± 1.9,
50µM – 44.3 ± 18.4 vs 6.6 ± 3.4
The amount of DOPAC obtained in effluent samples following Reserpine infusion showed a dose dependent increase in males and a dose dependent decrease in females.
Mean ± SEM DOPAC in pg/mg/min in Males
10µM – 27.4 ± 5.8, 20µM – 33.5 ± 6.4, 50µM – 63.7 ± 15.0
Mean ± SEM DOPAC in pg/mg/min in Females
10µM – 41.6 ± 3.9, 20µM - 36.3 ± 15.1, 50µM - 27.5 ± 8.7
These results demonstrate that significant gender differences are present in response to Reserpine suggesting that striatal vesicular function is different in males and females.
To further analyze these gender differences in the vesicular transporter mechanisms, we infused different concentrations of Reserpine (10µM, 20µM or 50µM) during superfusion of male (n=4) and female (n=4) corpus striatum tissue. After a three-interval basal collection period, the Reserpine solution was infused for a two collection interval period. The normal superfusion medium was resumed with the start of collection interval five and continued until the termination of the superfusion at collection interval eight.
The amount of DA obtained in effluent samples following Reserpine infusion showed a dose-dependent increase in males and were significantly greater compared with that from females at the 20µM and 50µM dose.
Mean ± SEM DA in pg/mg/min in Males vs Females:
10µM – 7.9 ± 4.3 vs 9.5 ± 2.5,
20µM – 20.4 ± 11.4 vs 2.9 ± 1.9,
50µM – 44.3 ± 18.4 vs 6.6 ± 3.4
The amount of DOPAC obtained in effluent samples following Reserpine infusion showed a dose dependent increase in males and a dose dependent decrease in females.
Mean ± SEM DOPAC in pg/mg/min in Males
10µM – 27.4 ± 5.8, 20µM – 33.5 ± 6.4, 50µM – 63.7 ± 15.0
Mean ± SEM DOPAC in pg/mg/min in Females
10µM – 41.6 ± 3.9, 20µM - 36.3 ± 15.1, 50µM - 27.5 ± 8.7
These results demonstrate that significant gender differences are present in response to Reserpine suggesting that striatal vesicular function is different in males and females.
Supported by NIH & APDA
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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