Neuroscience 2005 Abstract
| Presentation Number: | 188.2 |
|---|---|
| Abstract Title: | Cerebral blood volume mapping of diabetic mice with high-field strength MRI. |
| Authors: |
Ferrazzano, P.*1
; Small, S.1
; Vannucci, S.1
1Columbia Univ. Hospital, New York, NY |
| Primary Theme and Topics |
Homeostatic and Neuroendocrine Systems - Brain Blood Flow, Metabolism and Homeostasis -- Energy metabolism |
| Session: |
188. Energy Metabolism Poster |
| Presentation Time: | Sunday, November 13, 2005 9:00 AM-10:00 AM |
| Location: | Washington Convention Center - Hall A-C, Board # HH11 |
| Keywords: | diabetes, diabetic encephalopathy |
BACKGROUND: The brain has not traditionally been considered a target organ for complications of diabetes, however recent research suggests a duration dependent effect of diabetes on the central nervous system. Impaired neuronal function and decreased neuronal density in the hippocampus correlate with performance on behavioral tests in diabetic rats. Hippocampal cerebral blood volume (CBV), measured using MRI, has been correlated with neuronal function, neuronal glucose metabolism, and neuronal density in mice and humans. Using high field strength MRI, CBV maps of mouse hippocampal subregions can be created, providing unique insight into neuronal function.
OBJECTIVE: To create a CBV map characteristic of the early diabetic mouse brain. The noninvasive nature of this modality will allow us to follow the progression of neuronal dysfunction throughout the course of disease.
METHODS: Six week old mice are rendered diabetic by a 5 day treatment with Streptozotocin. Using a 9.4 Tesla vertical Bruker magnet, images are obtained prior to induction of diabetes, and again 1 week after induction of diabetes. Images are analyzed using MEDx image analysis software, and cerebral blood volume maps are generated, regions of interest are traced, and normalized CBV values are calculated for each hippocampal subregion.
RESULTS: A CBV map characterizing the early diabetic mouse brain was created. No statistical difference was found in CBV of hippocampal subregions in diabetic and control mice after 1 week of diabetes. These results establish the foundation for our ongoing work examining the CBV maps of diabetic mice after three months of diabetes, characterizing the alterations in regional CBV, and correlating these maps with histopathology.
OBJECTIVE: To create a CBV map characteristic of the early diabetic mouse brain. The noninvasive nature of this modality will allow us to follow the progression of neuronal dysfunction throughout the course of disease.
METHODS: Six week old mice are rendered diabetic by a 5 day treatment with Streptozotocin. Using a 9.4 Tesla vertical Bruker magnet, images are obtained prior to induction of diabetes, and again 1 week after induction of diabetes. Images are analyzed using MEDx image analysis software, and cerebral blood volume maps are generated, regions of interest are traced, and normalized CBV values are calculated for each hippocampal subregion.
RESULTS: A CBV map characterizing the early diabetic mouse brain was created. No statistical difference was found in CBV of hippocampal subregions in diabetic and control mice after 1 week of diabetes. These results establish the foundation for our ongoing work examining the CBV maps of diabetic mice after three months of diabetes, characterizing the alterations in regional CBV, and correlating these maps with histopathology.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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