Neuroscience 2005 Abstract
| Presentation Number: | 177.26 |
|---|---|
| Abstract Title: | Cloning and characterization of an unknown monoaminergic receptor in <i>Panulirus interruptus.</i> |
| Authors: |
Prince, E. H.*1
; Baro, D. J.1
1Biology, Georgia State Univ., Atlanta, GA |
| Primary Theme and Topics |
Sensory and Motor Systems - Invertebrate Sensory and Motor Systems -- Invertebrate motor systems |
| Secondary Theme and Topics | Sensory and Motor Systems<br />- Pattern Generation and Locomotion<br />-- Invertebrate pattern generation |
| Session: |
177. Invertebrate Motor Systems Poster |
| Presentation Time: | Sunday, November 13, 2005 9:00 AM-10:00 AM |
| Location: | Washington Convention Center - Hall A-C, Board # BB13 |
| Keywords: | GPCR, NEUROMODULATION, STOMATOGASTRIC, G PROTEIN |
The stomatogastric nervous system (STNS) of crustaceans offers several circuits that serve as models for central pattern generation. Many fundamental questions regarding monoaminergic neuromodulation have been addressed with this system. Though physiological responses to these modulators are well understood, their molecular mechanisms are only beginning to be revealed. Monoamines communicate with target neurons through highly conserved G protein coupled receptors (GPCRs). Database mining expeditions suggest that arthropods possess 18 monoamine receptors. Based on previous research manuscripts, these 18 receptors can be divided into 5 types: dopamine receptors (3), serotonin receptors (5), octopamine receptors (1), tyramine receptors (1), and unknown receptors (8). Preliminary characterizations for 3 of these unknown proteins were presented at the 2004 SFN meeting revealing a 4th dopamine receptor and 2nd and 3rd octopamine receptor. The remaining 5 unknown receptors have never been cloned or characterized from any arthropod species. In an effort to elucidate the molecular underpinnings of monoaminergic signal transduction in the STNS, we have cloned one of the unknown monoaminergic receptors from Panulirus interruptus. 601 amino acids long, it contains the characteristic seven transmembrane domains of GPCRS, producing three intracellular and extracellular loops. We compared the lobster amino acid sequence with the predicted protein from the Drosophila genome project database. The receptors were highly conserved with significant divergence occurring only in the amino terminus and intracellular loop three. NCBI BLAST searches indicate that this protein is most similar to adrenergic receptors. We are currently characterizing receptor function (G protein coupling and pharmacological profile) in a heterologous expression system and native membranes. Characterizing the localization, expression and function of this receptor in the STNS will significantly extend our understanding of the molecular basis of neuromodulation in this important model system.
Supported by NIH NS38770
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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