Neuroscience 2004 Abstract
| Presentation Number: | 106.9 |
|---|---|
| Abstract Title: | Use of bone marrow cells for spinal cord injury repair. |
| Authors: |
Sykova, E. M.*1,3
; Jendelova, P.1,3
; Urdzikova, L.1
; Burian, M.2,3
; Glogarova, K.1,3
; Hajek, M.1,2,3
1Dept Neurosci, Inst Exp Med, Prague 4, Czech Republic 2Czech Republic, ASCR, 142 20, 3ZRIR, ASCR, 142 20, |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Trauma -- Spinal cord |
| Secondary Theme and Topics | Motor Systems<br />- Spinal Cord<br />-- Damage and recovery |
| Session: |
106. Spinal Cord Injury: Transplantation I Poster |
| Presentation Time: | Saturday, October 23, 2004 1:00 PM-2:00 PM |
| Location: | San Diego Convention Center - Hall A-H, Board # WW6 |
| Keywords: | nanoparticles, MRI, stem cells, injury |
Bone marrow contains a population of pluripotent cells that have the capacity to migrate towards a lesion and induce or facilitate site-dependent differentiation in response to enviromental signals. Magnetic resonance imaging (MRI) provides a noninvasive method for studying the fate of transplanted cells in vivo. Rat bone marrow stromal cells (MSCs) were isolated by their adherence to plastic, expanded in vitro and labeled with superparamagnetic nanoparticles. After the induction of a balloon-induced spinal cord compression lesion, the rats received intravenously either MSCs (2 million cells, 7 days post-injury) or granulocyte colony-stimulating factor (G-CSF; 25-30 micrograms from day 7 to 11 post-injury). Lesioned animals grafted with MSCs or treated with G-CSF had higher scores in BBB testing than did control animals. Furthermore, they showed the recovery of sensitivity in their hind limbs using a plantar test. In MR images, the lesion populated by grafted cells appeared as a dark hypointense area. Prussian blue staining confirmed a large number of iron-containing cells and the lesion was considerably smaller than in control animals. Our study recently led to a clinical study in which 11 patients with spinal cord injury were treated with autologous bone marrow mononuclear cells administered systemically. All patients tolerated the cell therapy well, and there were no complications observed. The results will be evaluated at 3, 6 and 12 months after therapy. We conclude that there may be various ways in which bone marrow stem cells interact with host CNS tissue and that the mechanisms behind the observed beneficial effects in animals, as well as in patients, need to be further evaluated. Supported by AV0Z5039906, MSMT LN00A065, J13/98:111300004, GACR 304/03/1189.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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