Neuroscience 2004 Abstract
| Presentation Number: | 104.3 |
|---|---|
| Abstract Title: | Stimulation of neurite outgrowth by immunophilin ligands: Quantitative analysis by Cellomics ArrayScan. |
| Authors: |
Wood, A.*1
; Liu, D.1
; Mark, R.1
; Campos, S.1
; McIlvain, H. B.1
; Fennell, M.1
; Dunlop, J.1
; Graziani, E. I.2
; Pong, K.1
1Discovery Neurosci., Wyeth Res., Princeton, NJ 2NY, CN 8000, 08543, |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Ischemia -- Neuroprotection and tolerance |
| Session: |
104. Ischemia: Neuroprotection and Tolerance IV Poster |
| Presentation Time: | Saturday, October 23, 2004 3:00 PM-4:00 PM |
| Location: | San Diego Convention Center - Hall A-H, Board # UU20 |
| Keywords: | cortical neurons, dorsal root ganglia, neurotrophic, neuroprotection |
Immunophilins are a class of proteins that are known for their roles in mediating the immunosuppressive actions of FK506, cyclosporin A (CsA), and rapamycin. FK506 and rapamycin bind to the FK506 binding proteins (FKBPs), while CsA binds to the family of cyclophilins. Reports have shown that immunophilins are expressed in the brain and spinal cord and are 10-100 fold higher in CNS tissue than immune tissue. Furthermore, immunophilin expression is increased following nerve injury, suggesting potential therapeutic utility for immunophilin ligands. We have shown that treatment with FK506, FK520, CsA, or rapamycin for 72 hrs increases neurofilament immunoreactivity and survival in cultured cortical neurons (Liu et al., SFN 2004). In this study, we report the utility of the Cellomics ArrayScan to characterize and quantify neurite outgrowth stimulated by immunophilin ligands in cultured cortical neurons and F-11 cells (rat dorsal root ganglia x mouse neuroblastoma hybrid). Cultured cortical neurons were treated with FK506, FK520, CsA, or rapamycin for 72 hrs. F-11 cells were treated with FK506, FK520, CsA, or rapamycin for 96 hrs. Cultures were processed with a TUJ-1 antibody and neurite outgrowth was determined by using the Enhanced Neurite Outgrowth (ENO) algorithm with the Cellomics ArrayScan. The results showed that all four immunophilin ligands, in a dose-dependent manner, significantly increased both average and total neurite length in both cell types. Taken together, these results suggest the potential usefulness of the ENO algorithm and Cellomics ArrayScan to efficiently quantify neurite outgrowth stimulated by immunophilin ligands.
<B>Conflict of Interest:</B> All authors are employees of Wyeth.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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