Neuroscience 2004 Abstract
| Presentation Number: | 103.1 |
|---|---|
| Abstract Title: | Acute intracerebral hemorrhage (ICH) in the rat: Behavior, MRI, histopathology, immunohistochemistry, and albumin-neuroprotection. |
| Authors: |
Obenaus, A.*1
; Belayev, L.2
; Saul, I.2
; Zhao, W.2
; Busto, R.2
; Vigdorchik, A.2
; Ginsberg, M. D.2
1Dept Radiation Med., Loma Linda Univ, Loma Linda, CA 2FL, 11175 Campus St, 92354, |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Ischemia -- Neuroprotection and tolerance |
| Secondary Theme and Topics | Cognition and Behavior<br />- Human and Animal Cognition and Behavior<br />-- Learning & memory: Pharmacology |
| Session: |
103. Ischemia: Neuroprotection and Tolerance III Poster |
| Presentation Time: | Saturday, October 23, 2004 1:00 PM-2:00 PM |
| Location: | San Diego Convention Center - Hall A-H, Board # TT31 |
| Keywords: | ISCHEMIA, RAT, TRAUMA, BLOOD |
A comprehensive characterization of intra-striatal ICH produced in rats by the double-injection method (15 µL fresh arterial blood over 3 min, followed 7 min later by 30 µL over 5 min). Sequential susceptibility-weighted (SWI) and T2 MR imaging was carried out on a 4.7T MRI at 0-1, 6, 24, 72h, and 7 days after induction of ICH. Neurological status before ICH and after treatment (at 4, 24, 48, 72h and 7 days) was graded as previously described1. Following the final MRI study, brains were fixed and re-imaged on an 11.7T MRI and studied by histopathology and immunochemistry. SWI MRI revealed a consistent hematoma involving the striatum and overlying corpus callosum, with significant volume changes over time. Histological hematoma volume at 7d was 5.1±0.1 mm3 (SEM). Other findings included peripheral microglial activation and macrophage infiltration (ED-1), peripheral extracellular iron deposition and hemoxygenase-1 (HO-1) expression; and bilateral astrocytic activation (GFAP). The area of neuronal loss by Neu-N immunochemistry was 2-3-fold greater than the hematoma itself. Hematoma volume computed from T2 MRI images and from H&E histopathology agreed closely with one another and were highly correlated (p=0.002). SWI-MRI lesion volumes were also highly correlated to histological volumes (p<0.001) but overestimated histological hematoma volume by ~5-fold. Human albumin administration (25% solution, 1.25 g/kg i.v. at 50 min after ICH) led to a progressive neurobehavioral improvement over the subsequent 2-7-day survival period, compared to saline-treated ICH rats; scores at 7 days were 1.3±0.4 (albumin) vs. 3.8±0.4 (saline) (p<0.001). Histological and SWI-MRI lesion volumes were not affected by albumin.
1Belayev et al, Stroke, 27, 1616-1623, 1996.
1Belayev et al, Stroke, 27, 1616-1623, 1996.
Supported by NS05820, NS40406, and NASA Coop. Agreement NCC9-149
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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