Neuroscience 2005 Abstract
| Presentation Number: | 135.12 |
|---|---|
| Abstract Title: | Myelination defects assessed by diffusion tensor magnetic resonance imaging in jimpy mutant brain. |
| Authors: |
Harsan, L. A.*1
; Gonul, A. S.1
; Poulet, P.1
; Guignard, B.1
; Parizel, N.1
; Guiberteau, T.1
; Grucker, D.1
; Skoff, R. P-.2
; Ghandour, M.1
1Institut de Physique Biologique, Laboratoire de Neurobiologie In Vivo, Univ. Louis Pasteur, Strasbourg, France 2MI, 4 rue Kirscheleger, 67085, |
| Primary Theme and Topics |
Disorders of the Nervous System - Demyelinating Disorders -- Human studies and animal models |
| Secondary Theme and Topics | Techniques in Neuroscience<br />- Staining, Tracing, and Imaging Techniques |
| Session: |
135. Demyelinating Diseases Slide |
| Presentation Time: | Sunday, November 13, 2005 10:45 AM-11:00 AM |
| Location: | Washington Convention Center - Room 154A |
| Keywords: | DT-MRI, hypomyelination and recovery, sexual dimorphism, PLP/DM20 gene mutation |
Diffusion tensor magnetic resonance imaging (DT-MRI) is among the most promising noninvasive imaging techniques, extensively used in brain pathology assessment. Pelizaeus-Merzbacher Disease (PMD) is a disorder linked to a point mutation in PLP/DM20 gene on X chromosome producing an irreversible hypomyelination in affected males. The heterozygous females, carriers of the mutated gene exhibit a transient hypomyelination and oligodendrocyte loss, followed by myelin recovery. The jimpy mouse, a model of human PMD, was used for in vivo DT-MRI brain investigation. Both affected males and female carriers were used to analyze, noninvasively, the sexual dimorphism of white matter in this neurodegenerative disorder and to quantify the hypomyelination and recovery, correlated with histopathology.The myelin loss and regeneration were assessed by calculating and mapping the radial(D⊥) and axial (D‖) water diffusion to axonal tracts, fractional anisotropy (FA) and mean diffusivity. A significant increase of D⊥, caused by the lack of myelin was observed in the white matter of jimpy males. Interestingly, the great elevation of D⊥ was not accompanied by modifications in D‖, despite morphological changes of axons. The severe astrocytic hypertrophy observed in jimpy males may have impact on the water diffusion values found in hypomyelinated brains. Intermediate levels of D⊥ were detected in female carriers at 25 days, when compared with values of both control females and affected males, consistent with intermediate levels of myelination. Subsequently, D⊥ values decreases progressively with the gradual recovery of female white matter. FA maps display also low values in the hypomyelinated male brains, while in female carriers, FA values are reduced in a lesser degree and the progressive increase was associated with regeneration.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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