Neuroscience 2004 Abstract
| Presentation Number: | 64.6 |
|---|---|
| Abstract Title: | Monocyte chemoattractant protein-1 enhances excitability of nociceptive and non-nociceptive neurons in chronically compressed dorsal root ganglia. |
| Authors: |
Sun, J.*1
; Ma, C.1
; Tan, Z.1
; White, F. A.2
; Miller, R. J.3
; LaMotte, R. H.1
1Anesthesiol., Yale Univ Sch. Med., New Haven, CT 2IL, 333 Cedar Street, BML429, 06510, 3USA, 333 Cedar Street, BML429, 06510, |
| Primary Theme and Topics |
Sensory Systems - Pain -- Neuropathic pain |
| Secondary Theme and Topics | Sensory Systems<br />- Pain<br />-- Pain Models |
| Session: |
64. Neuropathic Pain Mechanisms Poster |
| Presentation Time: | Saturday, October 23, 2004 2:00 PM-3:00 PM |
| Location: | San Diego Convention Center - Hall A-H, Board # T6 |
| Keywords: | NEUROPATHIC PAIN, CHEMOKINE |
A chronic compression of the dorsal root ganglion (CCD) upregulates expression of monocyte chemoattractant protein-1 (MCP-1) and mRNA for the C-C chemokine receptor, CCR2, in subpopulations of neurons in the compressed ganglion (White et al; Waters et al. SFN abstrs. 2004). Because CCD produces hyperalgesia and increased neuronal excitability, and certain chemokines can excite dorsal root ganglion (DRG) neurons in culture (Oh et al. J. Neurosci. 21:5027), we explored the effects of MCP-1 on the excitability of neurons in the intact DRG with prior CCD or without (control). Intracellular recordings were obtained, in vitro, from visualized somata of small, medium and large size. Neurons were also categorized, in response to electrical stimulation of the dorsal root, by axonal conduction velocity and by the absence or presence of a hump on the falling phase of the evoked action potential (the hump is typical for nociceptive neurons recorded in vivo). MCP-1 (100 nM, 1 min duration) was topically applied to the soma of each recorded neuron. MCP-1 depolarized (>= 2 mV) only 2 Of 33 control DRG neurons (6%). In contrast, 25 of 37 CCD neurons (68%) responded to MCP-1 with a mean depolarization of 4.7±0.94 mV (range of 2.1 to 15.1 mV). MCP-1 induced discharges in 4 CCD neurons. Proportions of responsive small-, medium- and large-sized neurons were 6/10, 12/17 and 7/10, respectively. Responsive CCD neurons included 7 classified as nociceptive and 18 as non-nociceptive. MCP-1, in addition to its role in attracting monocytes and T lymphocytes may act as a proinflammatory cytokine by exciting nociceptive and non-nociceptive neurons thereby contributing to pain and hyperalgesia after CCD.
Supported by NIH grant NS14624, NS38317 and National MS Society
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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