Neuroscience 2004 Abstract
| Presentation Number: | 1029.13 |
|---|---|
| Abstract Title: | Mouse model of microcephaly as revealed by MRI. |
| Authors: |
Gleeson, J. G.*2
; Berdy, S.4
; Davis, E.2
; Yoder, E. J.1,3
1Dept Radiology, UCSD Sch. of Med., La Jolla, CA 2Dept Neurosci., UCSD Sch. of Med., La Jolla, CA 3Ctr. for Functional MRI, UCSD Sch. of Med., La Jolla, CA 4Grad. Program in Biomed. Sci., UCSD Sch. of Med., La Jolla, CA |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Developmental Disorders -- Genetic |
| Secondary Theme and Topics | Cognition and Behavior<br />- Human Cognition, Behavior, and Anatomy<br />-- Anatomy |
| Session: |
1029. Developmental Disorders: Mental Retardation II Poster |
| Presentation Time: | Wednesday, October 27, 2004 1:00 PM-2:00 PM |
| Location: | San Diego Convention Center - Hall A-H, Board # EEE31 |
| Keywords: | magnetic resonance microscopy, mouse phenotyping, cortical development, volumetric analysis |
Mouse models provide useful tools to examine the genetic basis of disease. The Tennessee Mouse Genome Consortium screen for clinically relevant mouse models identified one mutant (178TNC) with reduced brain weight that was 3-5 standard deviations below the mean. We sought to validate this preliminary result and precisely quantify differences in brain size between mutant and wild-type animals using anatomical MRI scans with volumetric analysis. Freshly fixed age-matched mouse heads were secured within a custom-built T/R TEM RF coil using a locking mouth bar unit, ensuring reproducible head orientation and stabilization. Images were acquired using a proton density-weighted spin echo pulse sequence (TR=5000, TE=8.6, bandwidth=30K, voxel size=100x100x350 microns) on a Magnex 7 Tesla MRI scanner driven by a Varian console running VNMRJ. “Whole brain” regions for analysis were traced in coded data sets using XVOXTRACE. These regions included the olfactory bulbs and cerebellum and were demarcated at the level of the peduncles. Volumes of the “whole brain” traced regions were calculated using ANALYZE. We found that the mutant mouse heads measured 9.02% lower volume as compared to wild-type heads, consistent in each orientation. While the exact mapping of the genetic mutation remains to be completed, the mutation is known to occur in a region altered in two human microcephaly syndromes. Thus, this mutant mouse strain may be a strong candidate for a model of microcephaly.
Supported by NIH (EB 03436 to EJY), Edwards Foundation (to JGG)
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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