Neuroscience 2003 Abstract
| Presentation Number: | 54.3 |
|---|---|
| Abstract Title: | Vesicular docking in mice expressing constitutively active H-rasG12V. |
| Authors: |
LeBoutillier, J. C.*1
; Marrone, D. F.1,2
; Kushner, S. A.
; Elgersma, Y.
; Silva, A. J.
; Petit, T. L.1,2
1Psychology, Univ. of Toronto, Toronto, Canada 2Neurosci., Univ. of Toronto, Toronto, Canada |
| Primary Theme and Topics |
Synaptic Transmission and Excitability - Synaptic Plasticity -- Other |
| Secondary Theme and Topics | Synaptic Transmission and Excitability<br />- Synaptic Transmission<br />-- Presynaptic mechanisms |
| Session: |
54. Neurotransmitter Release: Docking & Fusion I Poster |
| Presentation Time: | Saturday, November 8, 2003 3:00 PM-4:00 PM |
| Location: | Morial Convention Center - Hall F-I, Board # F7 |
| Keywords: | MAP KINASE, ERK, SYNAPTIC VESICLES, CA1 |
Ras, part of the mitogen-activated protein kinase (MAPK) pathway, is a protein expressed ubiquitously in eukaryotic cells which plays a critical role in transmitting extracellular signals. It has been shown that expression of the active RasG12V mutation in glutamatergic forebrain neurons of mice results in enhanced hippocampal-dependent learning and LTP at Schaffer collateral synapses. In addition, short-term synaptic depression was attenuated and paired-pulse facilitation was increased. We have also demonstrated that RasG12V mice have enhanced MAPK-dependent phosphorylation of synapsin I (SFN 2002 abstract # 779.9). Collectively, this implicates a potential function for Ras in presynaptic vesicle distribution. This possibility was addressed through serial electron microscopy of excitatory axospinuous synapses in CA1. Reconstructions of complete terminals and spines revealed a significant increase in the number of docked vesicles in RasG12V mice without any change in the size of the vesicular pool or terminal morphology. This further implicates Ras as a fundamental signaling molecule regulating presynaptic function.
Supported by NSERC
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
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