Neuroscience 2005 Abstract
| Presentation Number: | 1004.9 |
|---|---|
| Abstract Title: | Brain disorders in mouse model for brain ageing as evidenced by MRI and immunocytochemical analysis. |
| Authors: |
Borg, J.*1
; Sarda, N.2
1Lab Neurochimie, Faculte de Medecine, Saint Etienne, France 2France, Bd Pasteur, 42055, |
| Primary Theme and Topics |
Disorders of the Nervous System - Neurodegenerative and Movement Disorders -- Dementia: Experimental models |
| Session: |
1004. Cholinergic System, Down's Syndrome, and Signaling Poster |
| Presentation Time: | Wednesday, November 16, 2005 1:00 PM-2:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # OO29 |
| Keywords: | BRAIN IMAGING, HIPPOCAMPUS, NITRIC OXIDE, ACETYLCHOLINE |
The aim of study was to identify potential mechanisms of neurodegeneration by correlating image analysis of brain structures and immunocytochemical analysis of cholinergic pathways. The study was performed in transgenic mice that overexpress either normal human APP (amyloid precursor protein) or SOD1 (superoxyde dismutase 1) or a combination of both genes. Neuropathological studies were performed with T2 magnetic resonance imaging. Multislice axial and sagittal sequences were acquired in a Brucker Biospec (7 Tesla) with spin-echo imaging (TE 65ms, slice thickness 0.40 mm, data matrix 192x128). Following image analysis of these sequences, 3D construct of various brain structures were performed. Statistical comparison of brain regions volumes was performed between various mice.
As nitric oxide (NO) may affect cholinergic neurons development, we investigated the morphology of neurons expressing ChAT or NADPH-diaphorase in the brain of transgenic mice. Heavily stained neurons were found in cholinergic regions such as LDT, PPT, medial septum and in cerebral cortex. Striking differences in dendrites orientation could be seen between mice, that may reflect region–specific role of NO or ACh in brain alteration during ageing.
As nitric oxide (NO) may affect cholinergic neurons development, we investigated the morphology of neurons expressing ChAT or NADPH-diaphorase in the brain of transgenic mice. Heavily stained neurons were found in cholinergic regions such as LDT, PPT, medial septum and in cerebral cortex. Striking differences in dendrites orientation could be seen between mice, that may reflect region–specific role of NO or ACh in brain alteration during ageing.
Supported by Fondation J.Lejeune
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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