Neuroscience 2003 Abstract
| Presentation Number: | 941.8 |
|---|---|
| Abstract Title: | Norepinephrine release in cerebellum during delay classical eyeblink conditioning: a microdialysis study. |
| Authors: |
Paredes, D. A.*1
; Cartford, M. C.1
; Stellwagen, H.1
; Fister, M.1
; Bickford, P. C.1,2
1Ctr. of Excellence for Aging and Brain Repair, Univ. of South Florida, Tampa, FL 2FL, 12901 Bruce B. Downs Blvd, 33612, |
| Primary Theme and Topics |
Cognition and Behavior - Animal Cognition and Behavior -- Learning & memory: Pharmacology |
| Secondary Theme and Topics | Neurological and Psychiatric Conditions<br />- Behavioral Pharmacology<br />-- Monoamines and behavior |
| Session: |
941. Learning & Memory: Pharmacology VI Poster |
| Presentation Time: | Wednesday, November 12, 2003 4:00 PM-5:00 PM |
| Location: | Morial Convention Center - Hall F-I, Board # K10 |
| Keywords: | associative learning, cerebellum, motor learning |
Delay classical eyeblink conditioning is an important model of associative, cerebellar dependent learning. Our laboratory has been investigating the role of norepinephrine (NE) in this learning paradigm. We have demonstrated that blocking either the β-noradrenergic receptor (SFN 2003), adenylate cyclase or protein kinase A (SFN 2002) with local infusions into cerebellar lobule HVI and interpositus nucleus can interfere with learning. In this study our goal was to determine whether NE release is observed in HVI during delay eyeblink conditioning. Rats were prepared for in vivo microdialysis in order to monitor the NE levels prior to and during training trials. Three month old F344 rats were operated to place microdialysis probe guide cannulas into cerebellar lobule HVI and to anchor headstages with EMG eyelid monitoring wires to the skull. The night before training microdialysis probes were placed into cerebellar lobule HVI at a flow rate 0.1 ml/min. The following morning rats were placed into the eyeblink training chamber and the flow rate was increased to 2ml/min. Dialysate samples were collected every 10 minutes. After 5 baseline samples were collected rats received 50 training trials (45 paired with a 12 psi airpuff and 5 tone only [3 Khz 85 dB] trials), or Rescorla type pseudo conditioning. When rats were probed on either day 1 or day 2 of normal training we observed a significant release of NE during and after training. Rats receiving pseudo conditioning did not show significant changes in baseline NE release on day 1 or day 2 of conditioning trials.
Supported by NSF 0196474
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
Copyright © 2003-2026 Society for Neuroscience; all rights reserved. Permission to republish any abstract or part of any abstract in any form must be obtained in writing by SfN office prior to publication.
