Neuroscience 2002 Abstract
| Presentation Number: | 98.2 |
|---|---|
| Abstract Title: | ISCHEMIC PRECONDITIONING DOES NOT BLOCK CASPASE-3 ACTIVATION, BUT PREVENTS APOPTOTIC DNA FRAGMENTATION IN HIPPOCAMPAL CA1 PYRAMIDAL NEURONS. |
| Authors: |
Yokota, H.*1
; Tanaka, H.1
; Simionescu, M.1
; Jover, T.1
; Calderone, A.1
; Morosan, C.1
; Bennett, M. V. L.1
; Zukin, R. S.1
1Dept of Neurosci, Albert Einstein Coll of Med, Bronx, NY |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Ischemia -- Neuroprotection and tolerance |
| Session: |
98. Ischemia: neuroprotection and tolerance II Poster |
| Presentation Time: | Sunday, November 3, 2002 9:00 AM-10:00 AM |
| Location: | Hall A2-B3 Z-16 |
| Keywords: |
Animals subjected to sublethal transient global ischemia (ischemic preconditioning) exhibit neuroprotection against subsequent global ischemia-induced neuronal death in the hippocampal CA1 (ischemic tolerance). Preconditioning acts on apoptotic signaling cascades to prevent ischemia-induced upregulation of the pro-apoptotic neurotrophin receptor p75 and DNA fragmentation, as assessed by TUNEL staining in CA1 neurons. In contrast, preconditioning does not block ischemia-induced activation of caspase-9 or of caspase-3 (in accordance with H. Tanaka et al., SfN abstract, 2001). Caspase-3 activation has been considered a point of no return in apoptosis These findings indicate that preconditioning acts at a step downstream from caspase-3 activation, but upstream from apoptotic DNA fragmentation to protect CA1 neurons. Studies are underway to determine whether preconditioning prevents activation of DNA fragmentation factor 45 (DFF 45), an important target of activated caspase-3. Caspase-3 cleaves DFF 45 to generate activated DFF, which in turn mediates internucleosomal DNA fragmentation in post-ischemic neurons. These studies will determine whether ischemic preconditioning acts upstream or downstream to DFF cleavage to prevent apoptotic neuronal death.
Supported by NS31282
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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