Neuroscience 2003 Abstract
| Presentation Number: | 862.4 |
|---|---|
| Abstract Title: | Analysis of murine axon guidance mutants by MRI. |
| Authors: |
Ho, S. K. Y.*1
; Kovacev, N.2
; Chen, J.2
; Henkelman, M.2
; Henderson, J. T.1
1Fac. of Pharm., Univ. of Toronto, Toronto, Canada 2ON, 19 Russell St, M5S 2S2, |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, tracing and imaging techniques |
| Session: |
862. Staining, Tracing, & Imaging Techniques: Brain Imaging Poster |
| Presentation Time: | Wednesday, November 12, 2003 11:00 AM-12:00 PM |
| Location: | Morial Convention Center - Hall F-I, Board # VV39 |
| Keywords: | AXON GUIDANCE, IMAGING, CNS, MUTATION |
Histologic methods has long been used to examine tissues /organ systems for potential morphologic changes. However, the detailed examination of structures such as axon tracks, which can exhibit complex three-dimensional (3D) morphologies and cover significant distances, can be difficult to accurately determine using these methods. In order to gain a clearer understanding of morphologic ramifications which may occur following random or targeted genetic modification of specific murine lineages, we are developing MRI scanning and data analysis protocols which allow us to determine, in a relatively high throughput manner, changes in neural tracts within the murine CNS. In order to determine the practical limits of morphologic resolution using these MRI scanning and analysis protocols, we examined several axon guidance mutants previously characterized in our laboratory. The mutants examined represent members of the EphB family of receptor tyrosine kinases. T2 weighted multiple spin-echo images (TR 1500 ms, TE 30 ms, 60 μm,2 isotropic resolution) were determine for mutant and control specimens, using a 7 Tesla small-bore MRI unit. These parameters were chosen in order to optimize differences in relaxation times between gray and white matter components of the CNS. Following alignment to our universal coordinate system, specimens were compared with respect to structural morphologic differences. The results obtained demonstrate that the previously described changes in axon guidance seen for EphB null mice can clearly be observed using the MRI analysis system described; and furthermore indicate additional axon guidance defects for these mutants not previously described. Thus these data demonstrate the general utility of small-bore, high-field MRI has an effective morphologic tool for examination of the murine CNS.
Supported by Canadian Institute for Health Research, MDAC and ALS Cananda
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
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