Neuroscience 2003 Abstract
| Presentation Number: | 862.3 |
|---|---|
| Abstract Title: | Ex-vivo magnetic resonance imaging of β-amyloid plaques in transgenic AD mice. |
| Authors: |
Tang, C.*1,2
; Hajianpour, A.1
; Aguinaldo, G.1
; Ho, L.2
; Pasinetti, G.2
; Hof, P. R.3
; Perl, D. P.3,4
; Sadowski, M.7
; Wisniewski, T.5,6,7
1Radiology, Mt. Sinai Sch. of Med, New York, NY 2Psychiatry, Mt. Sinai Sch. of Med, New York, NY 3NeuroBiol., Mt. Sinai Sch. of Med, New York, NY 4Pathology, Mt. Sinai Sch. of Med, New York, NY 5NY, Mt. Sinai Sch. of Med., Dept Radiology, Box 1234 One Gustave L. Levy Pl NY,NY 10029, 10029, 6USA, Mt. Sinai Sch. of Med., Dept Radiology, Box 1234 One Gustave L. Levy Pl NY,NY 10029, 10029, 7Psychiatry, Mt. Sinai Sch. of Med., Dept Radiology, Box 1234 One Gustave L. Levy Pl NY,NY 10029, 10029, |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, tracing and imaging techniques |
| Secondary Theme and Topics | Neurological and Psychiatric Conditions<br />- Neurodegenerative Disorders<br />-- Alzheimer's Disease: APP, presenilin and Ab generation |
| Session: |
862. Staining, Tracing, & Imaging Techniques: Brain Imaging Poster |
| Presentation Time: | Wednesday, November 12, 2003 10:00 AM-11:00 AM |
| Location: | Morial Convention Center - Hall F-I, Board # VV39 |
| Keywords: | Imaging, APP, Microscopy |
According to the amyloid hypothesis, it is the progressive accumulation of β-amyloid that leads to a cascade of neurodegenerative processes in Alzheimer's disease (AD). Thus, current strategies for diagnosis and treatment evaluation rely on the ability to accurately quantify β-amyloid burden. It has previously been shown that β-amyloid plaques can be imaged using Magnetic Resonance Microscopy (MRM) at 40μm isotropic resolution in ex vivo human samples of the hippocampus. Transgenic (Tg) mice have been generated for research as β-amyloidosis models. Plaque sizes range can from 5μm to 200μm, with an average diameter of approximately 25μm. In the present study, we used high resolution MRM to explore the feasibility of visualizing β-amyloid plaque deposits in the brain of Tg2576 mice carrying the Swedish mutation of APP. We obtained T2 weighted 3D whole brain MRM data at 20μm and 25μm isotropic resolution. MRM images were compared with histological data to confirm that the signal seen on MRM corresponded to actual β-amyloid plaque deposits. We conclude that MRM is a practical and useful assay for imaging β-amyloid plaques with diameters as small as 20μm. These results will aid in the interpretation of MRI data gathered from in-vivo scans of mice, including scans wherein contrast agents are employed. This MRI technique can be easily applied to whole brain plaque quantification studies and for the purpose of studying treatment strategies using mouse models of AD, and may further be extended to in vivo studies tracking amyloid deposit formation and maturation throughout the animals life span.
Supported by HHMI Biomed Res. Sup. Prog. Grant, NIH AG05138, AG02219
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
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