Neuroscience 2001 Abstract
| Presentation Number: | 917.7 |
|---|---|
| Abstract Title: | IMMUNOCYTOCHEMICAL LOCALIZATION OF HEMICHOLINIUM-3-SENSITIVE CHOLINE TRANSPORTERS. |
| Authors: |
Apparsundaram, S.*1
; Savchenko, V.2,3
; Ferguson, S. M.2,3
; Wright, J.2,3
; Zwick, M.1
; Bauman, P. A.2,3
; Jain, N.4
; Kass, J. H.4
; Volpicelli, L. A.5
; Heilmann, C. J.5
; Levey, A. I.5
; Blakely, R. D.5
1Anatomy & Neurobiology, Univ. of Kentucky, Lexington, KY 2Pharmacology, Vanderbilt Univ., Nashville, TN 3Ctr. for Mol. Neurosci., Vanderbilt Univ., Nashville, TN 4Psychology, Vanderbilt Univ., Nashville, TN 5Neurology, Emory Univ., Atlanta, GA |
| Primary Theme and Topics |
Synaptic Transmission and Excitability - Transporters -- Monoamines, GABA, et3. |
| Secondary Theme and Topics | Synaptic Transmission and Excitability<br />- Neurotransmitters<br />-- Acetylcholine |
| Session: |
917. Transporters: other Poster |
| Presentation Time: | Thursday, November 15, 2001 10:00 AM-11:00 AM |
| Location: | Exhibit Hall F-20 |
| Keywords: | ACETYLCHOLINE, CHOLINERGIC, UPTAKE, ELECTRON MICROSCOPY |
The high-affinity, hemicholinium-3-sensitive choline transporter (CHT) is responsible for acquisition of choline as a precursor in the synthesis of acetylcholine (ACh). Previous autoradiographic and functional studies suggest a localization of CHT to cholinergic neurons and terminals. We have reported the cloning and characterization of human and mouse choline transporters (Apparsundaram et al. Biochem. Biophy. Res. Commun. 276:862-867, 2001 and SFN abstract 26:306.5, 2000). We have developed polyclonal and monoclonal antibodies to permit the localization of CHT protein in situ. Western analysis with affinity purified polyclonal antibodies identifies a single, 70 kDa species in mouse brain that carries N-glycosylation as revealed by PNGAse F digestion studies. Immunocytochemical studies using these antibodies along with probes for choline acetyltransferase (ChAT), vesicular ACh transporter (VAChT) or synaptophysin demonstrate labeling of cholinergic neurons in the striatum, basal forebrain and brainstem, and in cholinergic terminals in the CNS and PNS, including motor endplates. No labeling is evident of glia or noncholinergic neurons and CHTs could be identified in both rodent and primate brain. These studies identify new probes suitable for the identification of cholinergic pathways in situ and indicate a predominately presynaptic localization of CHT protein.
Supported by Univ. of Kentucky Intramural funds and the NIH
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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