Neuroscience 2001 Abstract
| Presentation Number: | 902.12 |
|---|---|
| Abstract Title: | ALTERED CEREBELLAR GRANULE CELL DEVELOPMENT AND PURKINJE CELL DENDRITIC MORPHOLOGY IN MICE WITH NMDA RECEPTOR 2C/2B SUBUNIT EXCHANGE. |
| Authors: |
Schlett, K.*1
; Metzger, F.2
; Herzog, K. H.3
; Pieri, I.4
; Eisel, U. L. M.4
1Univ., Budapest, Hungary 2Univ., Freiburg, Germany 3Tumorbiol. Center, Freiburg, Germany 4Univ., Stuttgart, Germany |
| Primary Theme and Topics |
Development - Synaptogenesis and Activity-Dependent Development -- Activity-dependent development and plasticity |
| Secondary Theme and Topics | Development<br />- Neurogenesis and Gliogenesis<br />-- Cell Migration |
| Session: |
902. Synaptogenesis and activity-dependent development: plasticity I Poster |
| Presentation Time: | Thursday, November 15, 2001 11:00 AM-12:00 PM |
| Location: | Exhibit Hall C-13 |
| Keywords: | CEREBELLUM, MIGRATION, GRANULE CELL, PURKINJE CELL |
N-methyl-D-aspartate receptors (NR) regulate neuronal plasticity, migration and excitotoxicity. The subunit composition thereby determines specific channel properties. In the postnatal cerebellum, the NR2B subunit is expressed in migrating granule cells and afterwards replaced by NR2C. We have examined the cerebellar development of transgenic mice (NR2CB) in which NR2C was exchanged by NR2B (see Eisel et al, SFN 2001).
In vivo histological analyzes indicated that the thickness of the external granule layer (EGL) was increased in NR2CB mice at P7 to P10. This tendency ceased by the second postnatal week. The thickness of the internal granule layer in NR2CB mice, on the other hand, was decreased from this time on. We then analyzed cerebellar development in slice cultures of P8 mice for up to 12 days in vitro (DIV). Immunostaining for TAG-1, an axonal marker expressed by migratory granule cells, was heavily detected up to DIV6 in wild-type slices and then declined progressively. At DIV12, only <20% of the slices were weakly TAG-1-positive. In contrast, >80% of the slices from NR2CB mice showed strong TAG-1 immunostaining. NeuN staining revealed that only 25% of the wild-type folia showed an EGL at DIV12 whereas in slices from NR2CB mice, >60% of all folia had a noticeable EGL. Furthermore, Purkinje cells from NR2CB mice developed a slightly larger but markedly less branched dendritic tree in the slice cultures.
The data suggest that in NR2CB mice granule cell migration - and probably survival - is retarded with consequences for the dendritic development of Purkinje cells.
In vivo histological analyzes indicated that the thickness of the external granule layer (EGL) was increased in NR2CB mice at P7 to P10. This tendency ceased by the second postnatal week. The thickness of the internal granule layer in NR2CB mice, on the other hand, was decreased from this time on. We then analyzed cerebellar development in slice cultures of P8 mice for up to 12 days in vitro (DIV). Immunostaining for TAG-1, an axonal marker expressed by migratory granule cells, was heavily detected up to DIV6 in wild-type slices and then declined progressively. At DIV12, only <20% of the slices were weakly TAG-1-positive. In contrast, >80% of the slices from NR2CB mice showed strong TAG-1 immunostaining. NeuN staining revealed that only 25% of the wild-type folia showed an EGL at DIV12 whereas in slices from NR2CB mice, >60% of all folia had a noticeable EGL. Furthermore, Purkinje cells from NR2CB mice developed a slightly larger but markedly less branched dendritic tree in the slice cultures.
The data suggest that in NR2CB mice granule cell migration - and probably survival - is retarded with consequences for the dendritic development of Purkinje cells.
Supported by DFG
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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