Neuroscience 2005 Abstract
| Presentation Number: | 840.9 |
|---|---|
| Abstract Title: | Cholinergic basal forebrain neurons containing vesicular glutamate transporter 3 project to the basolateral amygdala in rat. |
| Authors: |
Nickerson Poulin, A.*1
; Semba, K.1
; Mestikawy, S. E.2
1Dept Anat/Neurobiol, Dalhousie Univ., Halifax, Canada 2France, Sir Charles Tupper Med Bldg, 13th FL, B3H 1X5, |
| Primary Theme and Topics |
Neural Excitability, Synapses, and Glia: Cellular Mechanisms - Neurotransmitters and Signaling Molecules -- Acetylcholine: Anatomical localization |
| Secondary Theme and Topics | Neural Excitability, Synapses, and Glia: Cellular Mechanisms<br />- Transporters<br />-- Glutamate |
| Session: |
840. Cholinergic Modulation and Function Poster |
| Presentation Time: | Wednesday, November 16, 2005 8:00 AM-9:00 AM |
| Location: | Washington Convention Center - Hall A-C, Board # E47 |
| Keywords: | colocalization, confocal microscopy, retrograde tracing, acetylcholine |
The basal forebrain (BF) plays a major role in cortical and behavioural arousal, attention, as well as learning and memory. These processes are thought to be regulated by cholinergic, GABAergic and glutamatergic neurons that populate the BF and project widely through the neuraxis. These three neuronal groups may not be entirely exclusive to each other, and it has been proposed that some cholinergic BF neurons might co-release glutamate. Consistent with this suggestion, our previous study using dual immunofluorescence for choline acetyltransferase (ChAT) and vesicular glutamate transporter 3 (VGLUT3) in rat showed that ChAT+/VGLUT3+ neurons were present in the ventral pallidum (~36%), magnocellular preoptic area (~13%), substantia innominata (~25%) and magnocellular basal nucleus (~25%)[Poulin et al. SFN Abstr. 950.5, 2004]. In the same study we also showed that cholinergic axon terminals in the basolateral amygdaliod nucleus (BLA), but not in other targets of BF cholinergic projections, were co-immunoreactive to VGLUT3. The present study sought to confirm the source of ChAT+/VGLUT3+ innervation to the BLA by using the retrograde tracer Fluoro-Gold. Following tracer injections into the BLA, substantial proportions of retrogradely labeled ChAT neurons in the ventral pallidum (~52%), magnocellular preoptic area (~19%), substantia innominata (~31%) and magnocellular basal nucleus (~35%) were VGLUT3+. Taken together with our previous findings, the new findings indicate that cholinergic BF neurons that contain VGluT3 selectively project to the BLA and may therefore have the capacity to release both glutamate and acetylcholine in the amygdala.
Supported by CIHR, NSERC PGS-B
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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