Neuroscience 2000 Abstract
| Presentation Number: | 868.16 |
|---|---|
| Abstract Title: | Lithium ions increase cysteine string protein gene expression in NGF-differentiated PC12 cells. |
| Authors: |
Cordeiro, M. L.*1
; Umbach, J. A.1
; Gundersen, C. B.1
1Department of Molecular and Medical Pharmacology and The Crump Institute for Biological Imaging, UCLA School of Medicine, Los Angeles, |
| Primary Theme and Topics |
J. Disorders of the Nervous System and Aging - 147. Neuropsychiatric disorders: pharmacological agents |
| Secondary Theme and Topics | J. Disorders of the Nervous System and Aging<br />- 140. Neuropsychiatric disorders |
| Session: |
868. Neuropsychiatric disorders: pharmacological agents II Poster |
| Presentation Time: | Thursday, November 9, 2000 11:00 AM-12:00 PM |
| Location: | Hall G-J |
| Keywords: | CSP, Manic-Depressive, Secretion, Synaptic-Vesicle |
It has been half a century since lithium salts were introduced into modern pharmacotherapy to control the symptoms of manic-depressive illness. However, the mechanism of action of this cation remains elusive. We recently reported that cysteine string proteins (csps) are up-regulated in vivo in rat brain at therapeutically relevant concentrations of lithium (SFN Abstract 25,1066). Here, we extend these observations to an in vitro system, the adrenal pheochromocytoma cell line, PC12. These cells are a useful model for studying excitable cell function because they exhibit neuron-like properties when differentiated by nerve growth factor (NGF) and display regulated secretion of catecholamines. Our results revealed that lithium at 1 mM up-regulated csp gene expression in NGF-differentiated PC12 cells, whereas the same lithium treatment of undifferentiated cells did not alter csp gene expression. As with the in vivo findings, the expression of SY and SNAP-25 was not altered by this lithium treatment. Concentration-effect studies showed that even at 0.5 mM, lithium induced a significant increase in csp expression. Immunocytochemistry revealed csp accumulation both in the cell body and boutons of lithium-treated PC12 cells. Furthermore, we detected a significant increase (∼30%) in the evoked-release of [3H]-DA in lithium-treated cells. These data suggest that lithium modifies the depolarization-dependent behavior of NGF-differentiated PC12 cells and that this effect may be due to alterations of csp gene expression.
Supported by UCLA Dissertation Year Fellowship (MLC), Department of Molecular and Medical Pharmacology, and National Institutes of Health grant NS31934 (JAU).
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2000 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2000. Online.
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