Neuroscience 2005 Abstract
| Presentation Number: | 816.12 |
|---|---|
| Abstract Title: | Tracking of intravenously injected magnetically labelled progenitor cells in a mouse stroke model by high-field MRI. |
| Authors: |
Stroh, A.*1
; Zimmer, C.1,4
; Weir, K.1
; Gertz, K.2
; Kronenberg, G.2
; Sieland, K.1
; Werner, N.3
; Nickenig, G.3
; Mueller, S.2
; Endres, M.2
1Dept. of Neurorad., Charité Univ. Hospital, Berlin, Germany 2Dept. of Neurology, Charité Univ. Hospital, Berlin, Germany 3Germany, Schumannstr. 20/21, 10117, 4Dept. of Internal Med., Schumannstr. 20/21, 10117, |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, Tracing, and Imaging Techniques |
| Secondary Theme and Topics | Disorders of the Nervous System<br />- Ischemia<br />-- Human studies and animal models |
| Session: |
816. Imaging by MRI and PET III Slide |
| Presentation Time: | Wednesday, November 16, 2005 10:45 AM-11:00 AM |
| Location: | Washington Convention Center - Room 149A |
| Keywords: | Molecular Imaging, Cell Tracking, Ischemia |
Objectives - Recent studies suggest a positive role for stem cells in the regeneration of tissue following an ischemic insult. We hypothesize that spleen derived mononuclear cells (MNC’s) are actively recruited from the blood stream by signals released from ischemic brain tissue. In order to monitor migration of intravenously injected progenitor cells by high field MRI, we magnetically labeled primary culture spleen derived MNC’s with Very-Small-Superparamagnetic-Iron-Oxide-Particles (VSOP).
Materials and Methods – MNCs were isolated from transgenic GFP expressing mice and magnetically labelled with VSOP. 30 mice were treated with both 30 min and 60 min middle cerebral artery occlusion (MCAO) for stroke induction. 1 × 106 magnetically labelled cells were injected into the tail vein at various time points after MCAO. T2 and T2* weighted images were taken of mouse brains at 7T. For MR-histological correlation, Prussian Blue staining was performed for the magnetic label, and confocal microscopy for GFP fluorescence.
Results –Two days after cell treatment, several regions of shaded hypointense signal changes in the T2* weighted images appeared in the borderzone of the hyperintense (T2w) ischemic areas. Histologically, clusters of iron and GFP positive MNC’s were found to be closely correlating to the MR signal changes. Injection of MNC´s 8h after MCAO seemed to be most efficient.
Conclusion - The results show that in vivo tracking of systemically injected labelled progenitor cells appears possible by the use of high field MRI combined with an efficient labelling method. The T2* signal changes seem to reflect accumulations of systemically injected cells in the ischemic brain. Dynamics of cell recruitment can be visualized and different injection protocols have been evaluated.
Materials and Methods – MNCs were isolated from transgenic GFP expressing mice and magnetically labelled with VSOP. 30 mice were treated with both 30 min and 60 min middle cerebral artery occlusion (MCAO) for stroke induction. 1 × 106 magnetically labelled cells were injected into the tail vein at various time points after MCAO. T2 and T2* weighted images were taken of mouse brains at 7T. For MR-histological correlation, Prussian Blue staining was performed for the magnetic label, and confocal microscopy for GFP fluorescence.
Results –Two days after cell treatment, several regions of shaded hypointense signal changes in the T2* weighted images appeared in the borderzone of the hyperintense (T2w) ischemic areas. Histologically, clusters of iron and GFP positive MNC’s were found to be closely correlating to the MR signal changes. Injection of MNC´s 8h after MCAO seemed to be most efficient.
Conclusion - The results show that in vivo tracking of systemically injected labelled progenitor cells appears possible by the use of high field MRI combined with an efficient labelling method. The T2* signal changes seem to reflect accumulations of systemically injected cells in the ischemic brain. Dynamics of cell recruitment can be visualized and different injection protocols have been evaluated.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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