Neuroscience 2005 Abstract
| Presentation Number: | 816.7 |
|---|---|
| Abstract Title: | Simultaneous recording of cortical electrophysiological and hemodynamic responses to whisker stimulation during functional MRI of awake rabbits. |
| Authors: |
Talk, A. C.*1,2
; Li, L.2
; Iordanescu, G.2
; Weiss, C.1
; Disterhoft, J.1
; Wyrwicz, A.1,2
1Feinberg School of Medicine, Northwestern Univ., Chicago, IL 2IL, 1033 University Pl, Suite 100, 60212, |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, Tracing, and Imaging Techniques |
| Secondary Theme and Topics | Cognition and Behavior<br />- Animal Cognition and Behavior<br />-- Learning and memory: Physiology and imaging |
| Session: |
816. Imaging by MRI and PET III Slide |
| Presentation Time: | Wednesday, November 16, 2005 9:30 AM-9:45 AM |
| Location: | Washington Convention Center - Room 149A |
| Keywords: |
In an attempt to reconcile any differences between fMRI BOLD responses and neuronal activation, we have scanned the somatosensory cortex of awake rabbits during whisker stimulation, while simultaneously recording somatosensory cortical action potentials and field oscillations. Functional MR imaging experiments were performed on a 4.7T Bruker BioSpec imaging spectrometer operating at 200.3 MHz for 1H frequency. A single-turn, 40-mm diameter radiofrequency surface coil was used for both transmission and reception. Imaging data were acquired from four consecutive, 1mm-thick slices in transverse plane using a single-shot gradient-echo EPI pulse sequence with the following parameters: FOV=48mm x 48mm, image matrix=80 x 64, TR=2s and TE=20ms. All responses were detected using cross correlation, on a pixel-by-pixel basis, with a box function representing whisker stimulation timing. Neuronal activity was recorded through 100 μm PtIr electrodes chronically implanted into whisker cortex. Activity was amplified and differentially filtered to collect action potentials (band pass 300-3000 Hz) and local field potentials (band pass 40-150 Hz). Neuronal activity was analyzed after removal of blocks of gradient interference. Whisker-evoked spiking and local field activity occurred throughout the 20s whisker stimulation periods. Whisker-evoked BOLD responses also persisted throughout stimulation. The size of neuronal multi-unit responses and local field activity increased with increasing whisker stimulation frequency (from 10 to 18 Hz). The BOLD responses also increased in size and intensity with higher stimulation frequencies. Thus, these results indicate a correlation between the fMRI BOLD response and neuronal activation.
Supported by R01 NS44617, 1S10 RR15685
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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