Neuroscience 2005 Abstract
| Presentation Number: | 816.6 |
|---|---|
| Abstract Title: | Detection of brain functional column using activity-induced manganese-enhanced (AIM) MRI in the rat. |
| Authors: |
Kawai, Y.*1
; Aoki, I.2
; Shibata, S.1
; Mori, Y.1
; Umeda, M.2
; Higuchi, T.1
; Tanaka, C.1,2
1Neurosurgery, Meiji Univ. of Oriental Medicine, Kyoto, Japan 2Japan, Hiyoshi-cho, Funai-gun, 629-0392, |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, Tracing, and Imaging Techniques |
| Secondary Theme and Topics | Sensory and Motor Systems<br />- Tactile/Somatosensory<br />-- Whisker coding from afferents to cortex |
| Session: |
816. Imaging by MRI and PET III Slide |
| Presentation Time: | Wednesday, November 16, 2005 9:15 AM-9:30 AM |
| Location: | Washington Convention Center - Room 149A |
| Keywords: | MRI, BARREL, ACTIVATION, CORTEX |
Introduction: Each whisker corresponds to a single functional column in the Barrel field. In vivo observation of activation induced by whisker stimulation is important for the analysis of the functional linkage. Activity-Induced Manganese-enhanced (AIM) MRI is reported as a hemodynamics independent method in functional MRI study (Lin, et al.). Mn2+ enters neurons through voltage-gated Ca2+ channels during nerve action potential and leads to signal enhancement in active brain areas. The purpose of this study is to detect brain activation during whisker stimulation with high spatial resolution using hemodynamics independent AIM MRI.
Materials and Methods: Male SD rats were divided into 3 groups: normal control (n = 5), multi whiskers stimulation (n = 5), and single whisker stimulation (n = 5). Animals were prepared using previously described methods (Aoki I, NMR in Biomed. 2004, 17:1-12). The blood brain barrier was disrupted by 25% D-mannitol. Whisker E1 was stimulated with 2Hz frequency during the MnCl2 infusion. After the stimulation, T1-weighted images were acquired using 4.7T MRI (Bruker) with following parameters; TR/TE = 182/9.6 ms, FOV = 32 mm, and matrix size = 256*256.
Results and Discussion: Remarkable signal enhancement was observed in the S1BF area corresponding to sensory tactile stimulation in the vibrissa (Fig. 1). The S1BF area was significantly enhanced in both multi and single whisker stimulation groups in comparison with the control group (Fig. 2). Brain activation induced by whisker stimulation was successfully imaged in vivo using high resolution AIM MRI.
Materials and Methods: Male SD rats were divided into 3 groups: normal control (n = 5), multi whiskers stimulation (n = 5), and single whisker stimulation (n = 5). Animals were prepared using previously described methods (Aoki I, NMR in Biomed. 2004, 17:1-12). The blood brain barrier was disrupted by 25% D-mannitol. Whisker E1 was stimulated with 2Hz frequency during the MnCl2 infusion. After the stimulation, T1-weighted images were acquired using 4.7T MRI (Bruker) with following parameters; TR/TE = 182/9.6 ms, FOV = 32 mm, and matrix size = 256*256.
Results and Discussion: Remarkable signal enhancement was observed in the S1BF area corresponding to sensory tactile stimulation in the vibrissa (Fig. 1). The S1BF area was significantly enhanced in both multi and single whisker stimulation groups in comparison with the control group (Fig. 2). Brain activation induced by whisker stimulation was successfully imaged in vivo using high resolution AIM MRI.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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