Neuroscience 2005 Abstract
| Presentation Number: | 816.1 |
|---|---|
| Abstract Title: | Visualization of multimolecular events using RGB-composite manganese-enhanced MRI (MEMRI). |
| Authors: |
Aoki, I.*1
; Kawai, Y.1
; Umeda, M.1
; Higuchi, T.1
; Koretsky, A. P.2
; Tanaka, C.1
1Meiji Univ. Oriental Med, Kyoto, Japan 2MD, Hiyoshi-Cho, 629-0392, |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, Tracing, and Imaging Techniques |
| Secondary Theme and Topics | Techniques in Neuroscience<br />- Molecular and Genetic Techniques |
| Session: |
816. Imaging by MRI and PET III Slide |
| Presentation Time: | Wednesday, November 16, 2005 8:00 AM-8:15 AM |
| Location: | Washington Convention Center - Room 149A |
| Keywords: | BRAIN IMAGING, HIPPOCAMPUS, MRI, ACTIVATION |
Aim
Noninvasive observation and visualization of cellular and molecular events are important for understanding of brain function. Manganese-enhanced MRI (MEMRI) has recently been applied for various kinds of modalities such as for tract tracing, neuroarchitecture, neurotransmitter-induced neural activation (PhAIM), and functional MRI (AIM). These MEMRIs rely on many kinds of molecular and physiologic functions such as Ca channel opening, chemical-induced activation, and transport in the brain. The aim of this study was to reveal morphological and functional architecture of the hippocampus and develop new visualization methods that combine the use of the different modalities of MEMRI.
Methods
Three types of MEMRI were performed using SD rats. 1) Neuroarchitectural MEMRI (n=5): 75 mg/kg MnCl2 was IV administrated. 2) AIM MRI with (n=5) or without (n=5) whisker stimulation: The BBB was disrupted by mannitol injection. Whiskers were stimulated with 2Hz during the MnCl2 infusion. 3) PhAIM MRI for glutamate (n=5): Rats were prepared the same manner as the AIM. Glutamate were injected through the ECA. T1-weighted images were acquired on 4.7T MRI and composed to RGB map.
Results and Discussion
The RGB-composite MEMRI was produced (Fig.1). This map contains multiple molecular-physiologic information; Ca channel related neural activity (green), glutamate induced neural activity (blue), and neuroarchitectural data (red). Signal enhancement in the pyramidal cell layer was narrow only in AIM in comparison with the others (Fig.1, 2; arrows). The RGB-composite MEMRI is useful for evaluation between different kinds of molecular specific imaging such as MEMRI.
Noninvasive observation and visualization of cellular and molecular events are important for understanding of brain function. Manganese-enhanced MRI (MEMRI) has recently been applied for various kinds of modalities such as for tract tracing, neuroarchitecture, neurotransmitter-induced neural activation (PhAIM), and functional MRI (AIM). These MEMRIs rely on many kinds of molecular and physiologic functions such as Ca channel opening, chemical-induced activation, and transport in the brain. The aim of this study was to reveal morphological and functional architecture of the hippocampus and develop new visualization methods that combine the use of the different modalities of MEMRI.
Methods
Three types of MEMRI were performed using SD rats. 1) Neuroarchitectural MEMRI (n=5): 75 mg/kg MnCl2 was IV administrated. 2) AIM MRI with (n=5) or without (n=5) whisker stimulation: The BBB was disrupted by mannitol injection. Whiskers were stimulated with 2Hz during the MnCl2 infusion. 3) PhAIM MRI for glutamate (n=5): Rats were prepared the same manner as the AIM. Glutamate were injected through the ECA. T1-weighted images were acquired on 4.7T MRI and composed to RGB map.
Results and Discussion
The RGB-composite MEMRI was produced (Fig.1). This map contains multiple molecular-physiologic information; Ca channel related neural activity (green), glutamate induced neural activity (blue), and neuroarchitectural data (red). Signal enhancement in the pyramidal cell layer was narrow only in AIM in comparison with the others (Fig.1, 2; arrows). The RGB-composite MEMRI is useful for evaluation between different kinds of molecular specific imaging such as MEMRI.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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