Neuroscience 2001 Abstract
| Presentation Number: | 866.4 |
|---|---|
| Abstract Title: | Brain Tissue characteristics in vivo and post mortem in normal and lesioned rat brains analysed by MRI. |
| Authors: |
Carlsson, J.*1
; Suzuki, T.1
; Gulyas, B.1
; Her, S.2
; Bjelke, B.1
1Clin Neurosci, Karolinska Inst, Stockholm, Sweden 2Samba Sensors AB, Göteborg, Sweden |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Ischemia -- Cellular and molecular mechanisms |
| Secondary Theme and Topics | Motor Systems<br />- Cortex and Thalamus<br />-- Imaging |
| Session: |
866. Ischemia: cellular and molecular mechanisms XIII Poster |
| Presentation Time: | Wednesday, November 14, 2001 4:00 PM-5:00 PM |
| Location: | Exhibit Hall XX-21 |
| Keywords: | Animal model, Brain Imaging, Sensorimotor, Stroke |
The purpose of this report was to investigate the changes in brain tissue properties between in vivo and post mortem stage by using magnetic resonance imaging with a combination of T2, proton density (PD) and apparent diffusion coefficient (ADC) maps. Male Sprague rats, normal animals n=4, and one group n=3 with a pan-necrotic focal lesion in the sensori-motor cortex. The rats were recorded in a MR-scanner before, immediately after, 1h after death and in vitro after 2 d in formalin. The MRI-recordings were performed on a 4.7 T Bruker Biospec Avance 47/40 spectrometer. A double-echo RARE sequence was used. Seven contiguous coronal slices with regions of interest from Cortex, Thalamus and Striatum were chosen. Determination of T2 was made by multi-slice multi-echo MSME protocol. Determination of the ADC was made by conventional spin echo experiment. A PD increase of 12% in parallell with a decrease of 39% in T2-values was observed between 1 h after death to, in vitro, after 2 d in formalin. A decrease in ADC-values of 41% between in vivo and directly after death and 29% from 1 h after death to, in vitro, after 2 d in formalin. A decrease in T2-value reflect a less freedom of protons, an increased PD-value indicating in this specific situation a more spongy tissue containing more free water molecules and a decrease in ADC-values is a result in lost of perfusion and diffusion.Thus, MRI characterisation using T2-, PD- and ADC-maps could be a valuable tool in Post Mortem examination when trying to draw conclusions to a previous in vivo situation.
Supported by Konung Gustaf V:s och Drottning Viktorias Stiftelse, Karolinska Institutet Foundations
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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