Neuroscience 2001 Abstract
| Presentation Number: | 866.1 |
|---|---|
| Abstract Title: | MRI evaluation and Functional Assessment of Brain Injury after Hypoxic Ischemia in Neonatal Mice. |
| Authors: |
Lai, L. J.*1
; Dahlberg, V.2
; Fredholm, B. B.2
; ﾅd駭, U.2,3
; Chen, Z.1
; Bjelke, B.1
1Dept Clin Neuroscience, MR Research Center, Karolinska Inst, Stockholm, Sweden 2Physiology and Pharmacology, Karolinska Inst, Stockholm, Sweden 3Woman and Child Health, Karolinska Inst, Stockholm, Sweden |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Ischemia -- Cellular and molecular mechanisms |
| Secondary Theme and Topics | Motor Systems<br />- Cortex and Thalamus<br />-- Imaging |
| Session: |
866. Ischemia: cellular and molecular mechanisms XIII Poster |
| Presentation Time: | Wednesday, November 14, 2001 1:00 PM-2:00 PM |
| Location: | Exhibit Hall XX-21 |
| Keywords: | Animal model, Brain Imaging, Sensorimotor, Stroke |
Background and Purpose: Severe perinatal aphyxia is an important cause of brain injury in the newborn infant. We examined if the early events after hypoxic ischemia (HI) in the 7-day-old mouse brain detected by magnetic resonance imaging (MRI) were related to and the long-term functional effects assessed in the same animals at 4 weeks of age.
Methods: Hypoxic ischemia was induced in 7-day-old CD1 mice by exposure to 8% oxygen for 30 minutes after occlusion of the left common carotid artery. The resulting unilateral focal lesion was evaluated in vivo by MRI (T2 maps, ADC maps) at 3, 6, 24 hours and 5 days after hypoxia. Locomotion and sensorimotor function were analysed after 3 weeks. After 4 weeks morphological evaluation was performed on cresyl violet stained brain slices.
Results: A decrease in ADC-values in cortex on the affected side was found at 3 hours after reperfusion. A significant increase in T2-values was seen after 6h to 5 days. Maximal size of the lesion was attained at 3-6 hours after reperfusion and declined thereafter. Animals with MRI-detected lesions had a decreased forward locomotion, performed worse than controls in the beam-walking test and showed a unilateral hypotrophy in the cresyl-violet sections four weeks later.
Conclusions: The temporal progression of the damage after hypoxic ischemia in 7-day-old mice differs from that of the adult brain as judged by MRI. The early lesion detected by MRI was related to has functional impairments for these mice in near adult life.
Methods: Hypoxic ischemia was induced in 7-day-old CD1 mice by exposure to 8% oxygen for 30 minutes after occlusion of the left common carotid artery. The resulting unilateral focal lesion was evaluated in vivo by MRI (T2 maps, ADC maps) at 3, 6, 24 hours and 5 days after hypoxia. Locomotion and sensorimotor function were analysed after 3 weeks. After 4 weeks morphological evaluation was performed on cresyl violet stained brain slices.
Results: A decrease in ADC-values in cortex on the affected side was found at 3 hours after reperfusion. A significant increase in T2-values was seen after 6h to 5 days. Maximal size of the lesion was attained at 3-6 hours after reperfusion and declined thereafter. Animals with MRI-detected lesions had a decreased forward locomotion, performed worse than controls in the beam-walking test and showed a unilateral hypotrophy in the cresyl-violet sections four weeks later.
Conclusions: The temporal progression of the damage after hypoxic ischemia in 7-day-old mice differs from that of the adult brain as judged by MRI. The early lesion detected by MRI was related to has functional impairments for these mice in near adult life.
Supported by Konung Gustaf V:s och Drottning Viktorias Stiftelse, Karolinska Institutet Foundations
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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