Neuroscience 2003 Abstract
| Presentation Number: | 789.6 |
|---|---|
| Abstract Title: | Monitoring the migration of transplanted progenitor cells with PET and MRI. |
| Authors: |
Cicchetti, F.*1
; Bulte, J.2
; Owen, M.3,4
; Chen, I.4
; Lapointe, N.1
; Yu, M.4
; Wang, X.4
; Owen, C.4
; Jokivarsi, K.4
; Gross, R. E.
; Brownell, A.4
1Neurosci. unit, CRCHUL, Ste-Foy, Canada 2MD, 2705 Blvd. Laurier, G1V 4G2, 3USA, 2705 Blvd. Laurier, G1V 4G2, 4Sch. of Med., 2705 Blvd. Laurier, G1V 4G2, |
| Primary Theme and Topics |
Development - Neurogenesis and Gliogenesis -- Cell migration |
| Secondary Theme and Topics | Development<br />- Transplantation and Regeneration<br />-- Transplantation |
| Session: |
789. Transplantation and Regeneration: Transplantation III Poster |
| Presentation Time: | Wednesday, November 12, 2003 9:00 AM-10:00 AM |
| Location: | Morial Convention Center - Hall F-I, Board # C22 |
| Keywords: | STEM CELL, INFLAMMATION, PARKINSON, IMMUNOHISTOCHEMISTRY |
The aim of our study is to develop non invasive approaches to follow the fate, viability and migratory potential of progenitor cells following transplantation using super high resolution positron emission tomography (PET) and magnetic resonance imaging (MRI). We labeled rat subventricular zone (SVZ) progenitor cells and control cell line (PC12, pheochromocytoma cells) using 18F-fluorodeoxyglucose for PET studies and dendrimer-encapsulated super paramagnetic particles (Feredex) for MRI. These labeled cells were transplanted into the striatum or rostral migratory stream (RMS) of normal and 6-OHDA lesioned rats. During a period of 2 months, transplanted progenitor cells were easily identifiable with MRI. SVZ cells implanted into the RMS migrated after one week retention time toward olfactory bulb as seen with MRI. Slow migration of the cells implanted into the striatum was observed after 3 weeks. Histological analyses using Nissl, Iron stain, H&E and Fluoro-Jade, allowed delineation of the transplantation site and identification of viable cells. All transplanted animals showed graft survival in both RMS and striatum. Implanted PC12 cells developed massive tumors after one week retention time indicating cell survival as well as inflammation, detected by PET 11C-PK11195 (marker for activated microglia). SVZ cells did not develop tumors nor showed an inflammatory response. Ongoing histological analyses will reveal the phenotype of the transplanted cells and migratory distance achieved. These preliminary results demonstrate that cell viability and migration can be followed in vivo within individual animals for extended periods of time.
Supported by US Depart. of Energy
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
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