Neuroscience 2004 Abstract
| Presentation Number: | 792.8 |
|---|---|
| Abstract Title: | Widening the therapeutic window for stroke with human umbilical cord blood treatment in the middle cerebral artery occluded rat: Behavioral and physiological recovery. |
| Authors: |
Newcomb, J. D.*1,2
; Saporta, S.1,2,3
; Sanberg, P. R.1,2,3
; Willing, A. E.1,2,3
1Ctr. of Excellence for Aging and Brain Repair, Univ. of South Florida, Tampa, FL 2Depts. Neurosurgery, Univ. of South Florida, Tampa, FL 3Anat., Univ. of South Florida, Tampa, FL |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Ischemia -- Neuroprotection and tolerance |
| Session: |
792. Ischemia: Stem Cells and Gene Therapy Poster |
| Presentation Time: | Tuesday, October 26, 2004 4:00 PM-5:00 PM |
| Location: | San Diego Convention Center - Hall A-H, Board # VV10 |
| Keywords: | MCAO, HISTOCHEMISTRY, TRANSPLANTATION, MOTOR ACTIVITY |
Intravenous delivery of human umbilical cord blood (hUBC) cells in rat models of middle cerebral artery occlusion (MCAO) improves performance on tests of motor coordination when given 24 hrs after MCAO (Chen et al., Stroke 2001; Willing et al., J Neurosci Res 2003). Though this treatment time is sufficient for noticeable recovery there is in vitro evidence that delivery of hUCB between 48 and 72hrs may be more effective (Newman et al., SFN 2003). Astrocytic and microglial inflammatory responses to ischemia are at their peak at this time as are signals that attract intravenously administered hUCB cells to the site of injury. In the present study we set out to determine the optimal time at which delivery of hUCB was most beneficial for behavioral and neurological recovery. Sixty Sprague Dawley rats were transplanted 3hrs to 1mo post MCAO. MCAO-only rats served as a control. All rats were tested on a series of behavioral tests prior to stroke and again at 2 and 4wks post transplant. Similar to our previous observations we found improved behavioral recovery with hUCB treatment. Rats that received transplants 48hrs after MCAO showed motor coordination improvement in the rotorod test, reduction in motor asymmetry in the step and elevated body swing tests and decreased hyperactivity in locomotor measurements compared to control or other treatment times. Histological analysis paralleled the behavioral findings, revealing decreased lesion volume in the 48hr transplant time point. These results suggest that effective treatment for stroke may be possible up to 48hrs after the event.
Supported by Florida Biomedical Program (AEW, BM039)
<B>Conflict of Interest:</B> SS and AEW are consultants to Saneron CCEL Therapeutics, Inc. PRS is a co-founder of Saneron CCEL Therapeutics
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
Copyright © 2004-2025 Society for Neuroscience; all rights reserved. Permission to republish any abstract or part of any abstract in any form must be obtained in writing by SfN office prior to publication.
