Neuroscience 2001 Abstract
| Presentation Number: | 852.22 |
|---|---|
| Abstract Title: | In vivo, Trans-synaptic Neuronal Tract Tracing from the Murine Striatum and Amygdala Utilizing Manganese Enhanced MRI (MEMRI). |
| Authors: |
Pautler, R. G.*1
; Mongeau, R.1
; Jacobs, R. E.1
1Biology, Cal-Tech, Pasadena, CA |
| Primary Theme and Topics |
Cognition and Behavior - Animal Cognition and Behavior -- Learning & memory: Physiology and imaging |
| Secondary Theme and Topics | Motor Systems<br />- Basal Ganglia<br />-- Anatomy |
| Session: |
852. Animal cognition and behavior: learning and memory--physiology and imaging V Poster |
| Presentation Time: | Wednesday, November 14, 2001 2:00 PM-3:00 PM |
| Location: | Exhibit Hall TT-31 |
| Keywords: | MRI, amygdala, basal ganglia, mouse |
It has previously been shown that Mn2+ is taken up through Ca2+ channels and can be transported along axons. Because Mn2+ ion is also paramagnetic, these combined properties of Mn2+ allow for the use of the ion as an effective MRI detectable, neuronal tract tracer. A previous study has demonstrated Mn2+ tract tracing along the olfactory and visual pathways in live mice. Whereas the previous study traced from sensory pathways leading into the brain, the purpose of the present study was to delineate tract tracings from focal points located within the brain. Nanoliter injections of 5 mM MnCl2 into either the striatum or the amygdala demonstrated contrast enhancement along the correct neuronal circuitry. Furthermore, enhancement of homotopic connections was observed in both cases. In the amygdala tracings, a total of six synapses was traversed as evidenced by positive contrast enhancement due to Mn2+ accumulation (figure). Ten days post injection, the Mn2+ appeared to wash out as enhancement within the brain was not observed. Incorporation of this refined methodology should allow for repeated in vivo mapping of neuronal circuitry in behavioral animal paradigms.
Supported by the Human Brain Project (DA08944), the NCRR (RR13625), and the NIMH (MH61223)
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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