Neuroscience 2002 Abstract
| Presentation Number: | 875.8 |
|---|---|
| Abstract Title: | Estrogen regulation of CB1 cannabinoid receptor binding in the hippocampus. |
| Authors: |
Daniel, J. M.*1
; Winsauer, P. J.1
; Paul, D.1
; Moerschbaecher, J. M.1
1Department of Pharmacology and Experimental Therapeutics, Lousiania State University Health Sciences Center, New Orleans, LA |
| Primary Theme and Topics |
Cognition and Behavior - Animal Cognition and Behavior -- Learning & memory: Pharmacology |
| Session: |
875. Animal cognition and behavior: learning and memory--pharmacology: other Poster |
| Presentation Time: | Thursday, November 7, 2002 11:00 AM-12:00 PM |
| Location: | Hall A2-B3 P-3 |
| Keywords: | AUTORADIOGRAPHY, LEARNING AND MEMORY, RAT, FEMALE |
We have previously demonstrated that estrogen replacement in ovariectomized rats attenuates behavioral deficits produced by the cannabinoid receptor agonist, Δ9-THC, on a task of learning and memory. In the present experiment we determined if a similar regimen of estrogen replacement would affect levels of cannabinoid receptor binding in the hippocampus. Female rats, age 75 days, were ovariectomized and received subcutaneous implants of 5-mm Silastic capsules containing 100% cholesterol (n = 10) or 25% estradiol diluted in cholesterol (n = 10). Ten days after receiving the capsules, animals were sacrificed and frozen coronal sections (20 µm) were taken through the hippocampus. Sections were incubated with [3H]CP55,940 (10 nM) in order to selectively label CB1 receptors and were exposed to storage phosphor screens for 3 days. Quantitative autoradiography using phosphorimaging technology revealed a significant estrogen-induced downregulation of CB1 cannabinoid receptor density in the CA1 region of the hippocampus. No significant changes in levels of cannabinoid receptor density were revealed in CA3 or the dentate gyrus. These results indicate that the decreased sensitivity to the disruptive effects of a cannabinoid agonist on a task of learning and memory in ovariectomized rats undergoing estrogen replacement may be related to the ability of estrogen to influence CB1 cannabinoid receptors in CA1 of the hippocampus.
Supported by DA1416201
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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