Neuroscience 2005 Abstract
| Presentation Number: | 784.13 |
|---|---|
| Abstract Title: | Detection of gadolinium-enhanced lesions by MRI at 17 months postinjury in a rat hemisection spinal cord injury model: a longitudinal study. |
| Authors: |
Fricke, S. T.*1
; Finn, T. P.1
; Dai, H.1
; Eliva, L.1
; Slotkin, J.1
; Bregman, B. S.1
1Neuroscience, Georgetown Univ. Med Ctr., Washington, DC |
| Primary Theme and Topics |
Sensory and Motor Systems - Spinal Cord -- Injury and recovery |
| Secondary Theme and Topics | Techniques in Neuroscience<br />- Staining, Tracing, and Imaging Techniques |
| Session: |
784. Spinal Cord Injury: Cervical Injury Poster |
| Presentation Time: | Tuesday, November 15, 2005 1:00 PM-2:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # SS70 |
| Keywords: | MRI, LESION, IMAGING, ANIMAL MODEL |
MRI is used to monitor longitudinal changes in CNS morphology due to injury. Gadolinium (Gd) is the standard MRI contrast agent used for CNS injury in humans. We tested the ability of gadolinium to enhance lesion contrast on lesion progression from acute to chronic time points. Animals were imaged at 5 days, 3 weeks, 7, 15, and 17 months post injury (C4), and 3 days, 4 weeks, 6, 7, 15, 16, and 17 months post injury (T9). Animals were imaged with a 7.0 Tesla horizontal bore Bruker (Bruker Biospin, Billerica, MA) via 3D Turbo RARE (FOV 7.68X2.56X2.56 cm, Matrix 512X256X256, TR 800 ms, TE 8.2 ms, RARE factor 2). Animals were anesthetized with isolflurane 1.5%, tail vein injected with 1.5 ml Omniscan (gadodiamide, Amersham Health, Princeton, NJ), then placed in a 72mm birdcage volume coil. No implanted coils were used for this study. Comparisons at different timepoints clearly revealed the lesion presence at all time points. Lesions were significantly Gd enhanced, even at 17 months. Apparent lesion volume changed with time yet was still prominent at 17 months. At 17 months animals were sacrificed, brains and spinal cord collected intact, and the tissues pinned to thin sheets of dental wax and immersed in FOMBLIN (Ausimont, Bollate, Italy). Ex vivo images of each spinal cord were collected using identical in-vivo imaging protocols. The lesion area detected by ex-vivo imaging correlated well with the gadolinium enhanced lesions obtained from live animals. Histological and immunocytochemical evaluation of tissue sections collected from the same animals is presented comparing the apparent Gd enhanced MRI images with histological findings of anatomical changes. These findings support the proof of concept for using non-invasive MRI to follow changes within spinal cord lesion areas in long-term longitudinal studies.
Supported by This work was supported by NIH NS27054, NS050141 and the Wadsworth foundation.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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