Neuroscience 2000 Abstract
| Presentation Number: | 828.12 |
|---|---|
| Abstract Title: | A frameless stereotaxic MRI technique for placing neural recording electrodes. |
| Authors: |
Dubowitz, D. J.*1
; Christianson, G. B.1
; Boline, J.1
; Pezaris, J. S.1
; Andersen, R. A.1
1Biology 216-76, California Institute of Technology, Pasadena, CA |
| Primary Theme and Topics |
G. Motor Systems and Sensorimotor Integration - 86. Cortex and thalamus |
| Session: |
828. Cortex and thalamus: synchrony and analyses Poster |
| Presentation Time: | Thursday, November 9, 2000 11:00 AM-12:00 PM |
| Location: | Hall G-J |
| Keywords: | Magnetic resonance imaging, electrode mapping, monkey |
We describe a frameless stereotaxic method for guiding recording electrode position. This involves two steps; Stereotaxic mapping of the brain to position a recording chamber, and subsequent stereotaxic mapping of the brain beneath the chamber expressed in chamber coordinates. Prior to surgery the animal undergoes T1 weighted 3D MR imaging, providing an anatomical dataset of 0.7mm resolution. The monkey is anesthetized for the MRI using a cocktail of ketamine, xylazine and atropine. Only light anesthesia is needed as the method requires no rigid stereotaxic fixation. Images are post-processed using AFNI software. The 3D image is rotated into the standard stereotaxic plane using skull/brain anatomy, and stereotaxic zero is set in the midline at the extended horizontal convergence of the external auditory meatii (to coincide with the surgical stereotaxic unit). This MRI is used to determine the optimal center and angle for surgically placing the recording chamber. Following attachment of the chamber the monkey undergoes a second MRI with the recording chamber filled with Gd-DTPA. During post-processing the 3D dataset is again rotated, but this time so the z-axis corresponds to the long axis of the recording chamber. Using AFNI software, the location of a cortical area can be read off the MRI image, and the desired x,y,z position set into the electrode microdrive. We have validated this technique by making multiple recordings either side of the intraparietal sulcus in an awake behaving macaque. We were able to accurately place a recording electrode into area 5 and area 7 with an accuracy of less than 1 mm.
Supported by Sloan Foundation, National Eye Institute, ONR & NSERC
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2000 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2000. Online.
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