Neuroscience 2002 Abstract
| Presentation Number: | 857.3 |
|---|---|
| Abstract Title: | EFFECT OF CANNABINOIDS ON EXCITATION-CONTRACTION COUPLING IN FAST SKELETAL MUSCLE FIBERS OF THE FROG. |
| Authors: |
Velasco, R.*1
; Vasquez, C.1
; Trujillo, X.1
; Huerta, M.1
1CUIB, Universidad de Colima, Colima, Mexico |
| Primary Theme and Topics |
Motor Systems - Muscle and Motor Unit |
| Secondary Theme and Topics | Motor Systems<br />- Muscle and Motor Unit |
| Session: |
857. Muscle and motor unit: compartments and innervation Poster |
| Presentation Time: | Thursday, November 7, 2002 10:00 AM-11:00 AM |
| Location: | Hall A2-B3 H-8 |
| Keywords: | cannabinoids, skeletal muscle |
Cannabinoids derive from D-9 tetrahydrocannabinol, the active principle of marihuana. Although it is known that cannabinoids reduce motor activity at the central level in humans as well as in animals, little information exists about their direct effects on skeletal muscle. By means of isometric-tension recording, in fast skeletal muscle fibers obtained from the EDL digiti IV of Rana pipiens, we have analyzed the effect of the cannabinoid agonist WIN 55,212-2 (WIN) on the contractile responses that follow depolarization evoked by chloride-free high-potassium solution. The normal saline was (in mM): NaCl, 117.5; KCl, 2.5; CaCl2, 1.8; with imidazol-chloride 2.0 mM being added to adjust the pH to 7.4. Chloride-free high-potassium solution (40 mM) was prepared similar the above solution by an equimolar replacement of sodium by potassium and by substituting the chloride ions with an osmotically equivalent amount of gluconate. The experiments were performed at room temperature (22-24 °C). The effects of WIN(10-50 mM) were recorded 3 min after its application. The results showed a slowing of the time course of contracture activation by 50± 15% with respect to the control (n=3), and reductions in peak and total tensions of 10± 2.4% of control (n=3) and 10 ± 3.1% of control (n=4), respectively. These effects were reversed by washing out the drugs. These results suggest that the generation of isometric tension may be modified by the cannabinoid drugs partly by altering calcium release from the sarcoplasmic reticulum and probably by reducing the total intracellular concentration of this ion.
Supported by U. de Colima to C. Vasquez and MH (F R ABA-2002) and from FOMES-00 to XT and MH.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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