Neuroscience 2000 Abstract
| Presentation Number: | 814.11 |
|---|---|
| Abstract Title: | ;CD9-Tetrahydrocannabinol induces immediate-early gene expression in the striatum that depends on the ERK/MAPK signaling pathway: role of DA transmission. |
| Authors: |
Valjent, E.*1
; Pagès, C.1
; Rogard, M.1
; Besson, M. J.1
; Caboche, J.1
1Laboratoire de Neurochimie-Anatomie-CNRS-UMR7624, Paris, France |
| Primary Theme and Topics |
D. Neurotransmitters, Modulators, Transporters, and Receptors - 54. Cannabinoids |
| Secondary Theme and Topics | C. Excitable Membranes and Synaptic Transmission<br />- 33. Synaptic plasticity |
| Session: |
814. Cannabinoids: receptor actions Poster |
| Presentation Time: | Thursday, November 9, 2000 10:00 AM-11:00 AM |
| Location: | Hall G-J |
| Keywords: | addiction, synaptic plasticity, cannabinoids, basal ganglia |
The biochemical substrates underlying rewarding effects of Δ9-tetrahydrocannabinol (THC) are poorly understood. We analyzed intracellular signaling pathways mediating immediate early gene expression (c-Fos and Zif 268) after acute administration of THC in striatal neurons. In male CD1 mouse brain, immunohistochemical detection of phosphorylated ERK/MAPK proteins showed a progressive but transient activation of this pathway in the striatum. Activation of ERK occurred in both striatal neurons and the neuropil surrounding these activated neurons, suggesting a pre- and post-synaptic activation of this signaling pathway. A systemic injection of a specific inhibitor of ERK/MAPK (SL 327) prevented THC-induced expression of c-Fos and Zif 268. As previous reports show that THC induces DA release in the striatum, we then analyzed whether DA transmission played a role in these molecular events. We found that injection of a selective antagonist of DA-D1 receptors (SCH 23390), prior to THC administration, prevented ERK activation and c-Fos and Zif 268 expression in striatal neurons. The NMDA glutamate receptors antagonist, MK 801 abolished ERK activation in striatal neurons only. THC-induced gene expression was also abolished in MK 801 injected mice. These data suggest that the ERK/MAPK signaling pathway is involved in the long-term response underlying THC-rewarding effects in striato-nigral neurons. They also strongly support a role of DA transmission in THC-induced ERK activation and gene expression.
Supported by Centre National de la Recherche Scientifique (CNRS) and Université Pierre et Marie Curie.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2000 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2000. Online.
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