Neuroscience 2000 Abstract
| Presentation Number: | 814.4 |
|---|---|
| Abstract Title: | A cannabinoid receptor in <I>Drosophila</I> is pharmacologically unique. |
| Authors: |
Howlett, A. C.*1
; Mukhopadhyay, S.1
; Wilken, G. H.1
; Neckameyer, W. S.1
1Dept. Pharmacol. Physiol. Sci., St. Louis Univ., St. Louis, |
| Primary Theme and Topics |
D. Neurotransmitters, Modulators, Transporters, and Receptors - 54. Cannabinoids |
| Secondary Theme and Topics | G. Motor Systems and Sensorimotor Integration<br />- 95. Invertebrate motor and sensory systems |
| Session: |
814. Cannabinoids: receptor actions Poster |
| Presentation Time: | Thursday, November 9, 2000 11:00 AM-12:00 PM |
| Location: | Hall G-J |
| Keywords: | THC, G Protein Coupled Receptor, Signal Transduction, Insect |
Cannabinoid receptors have been characterized as two subtypes in mammalian species: CB1 (neuronal) and CB2 (immune). Using an antibody against the N-terminal 14 amino acid domain of the rat/human CB1 receptor, Western blots of Drosophila melanogaster heads revealed a CB1 receptor-like immunoreactive band at 105 kDa. Immunoreactive neurons were identified in the third instar larval and adult brains. The CB receptor agonist radioligand [3H]CP55940 quantitated specific binding in membranes to be 3-fold greater density in heads than in bodies. Heterologous displacement defined potency ratios as: CP55940 > desacetyllevonantradol, Δ9-tetrahydrocannabinol > arachidonylethanolamide. No displacement of [3H]CP55940 by aminoalkylindoles WIN55212-2 or WIN58000, or by aryl pyrazoles SR141716A or SR144528 was observed. Desacetyllevonantradol stimulated [35S]GTPγS binding to G proteins in head membranes under defined ionic conditions; however, this was not antagonized by SR141716A or SR144528. Thus, the recognition site for ligands of the CB-like receptor in Drosophila differs in its pharmacological profile from mammalian CB1 receptors.
Supported by NIH DA03690, DA06312 and NSF IBN-9816566.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2000 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2000. Online.
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