Neuroscience 2000 Abstract
| Presentation Number: | 813.2 |
|---|---|
| Abstract Title: | Anandamide sensitivity of C-terminal mutant AMPA receptors. |
| Authors: |
Akinshola, B. E.*1
; Wang, H.1
; Taylor, R. E.1
; Onaivi, E. S.2
1Pharmacol Dept, Howard Univ Col Med, Washington, DC 2Dept of Psychiatry , Vanderbilt Univ Sch Med, Nashville, TN |
| Primary Theme and Topics |
D. Neurotransmitters, Modulators, Transporters, and Receptors - 54. Cannabinoids |
| Secondary Theme and Topics | C. Excitable Membranes and Synaptic Transmission<br />- 34. Ligand-gated ion channels |
| Session: |
813. Cannabinoids: anandamide Poster |
| Presentation Time: | Thursday, November 9, 2000 9:00 AM-10:00 AM |
| Location: | Hall G-J |
| Keywords: | KAINIC ACID, VOLTAGE CLAMP, GLUTAMATE, CURRENT |
The endocannabinoid, anandamide (known to mimic the actions of Δ 9 THC in vivo), has been shown to modulate CNS receptors, including AMPA glutamate receptors in vivo, in a presynaptic fashion. Anandamide has also been shown to inhibit CNS receptors, including the recombinant AMPA glutamate receptors in vitro, independently of the cannabinoid receptor system. In order to probe the molecular mechanisms of anandamide inhibition of AMPA receptor function, we used the two-electrode voltage-clamp technique, to study Xenopus laevis oocyte expression of wild-type and c-terminal mutant AMPA receptors and their sensitivities to anandamide in vitro. The mutations targeted phosphorylation sites at the intracellular c-terminal domain of the AMPA receptor. The mutant receptors studied are S831A, T840A, S845A and S831A/S845A. Our results show that mutant receptors were significantly, less sensitive to anandamide inhibition than the wild-type GluR1 receptor subunit. The sensitivity differences between the mutants and the wild-type receptor subunit to anandamide may be indicative of the importance of phosphorylated amino acids as anandamide allosteric modulatory sites on the AMPA receptor. Supported by HU grant # NF99/02.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2000 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2000. Online.
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