Neuroscience 2001 Abstract
| Presentation Number: | 816.8 |
|---|---|
| Abstract Title: | Manganese-enhanced 3d mri of rat retinal projections in vivo. |
| Authors: |
Michaelis, T.*1
; Watanabe, T.1
; Fuchs, E.2
; Frahm, J.1
1Biomedizinische NMR Forschungs GmbH, Gottingen, Germany 2German Primate Center, Gottingen, Germany |
| Primary Theme and Topics |
Sensory Systems - Anatomy |
| Secondary Theme and Topics | Development<br />- Development of Sensory, Motor and Limbic Systems<br />-- Visual system |
| Session: |
816. Tactile/somatosensory: novel techniques Poster |
| Presentation Time: | Wednesday, November 14, 2001 4:00 PM-5:00 PM |
| Location: | Exhibit Hall F-69 |
| Keywords: | NMR, Axonal Transport, Animal model, Visual pathway |
Based on its neuronal uptake and axonal transport in analogy to calcium ions, manganese (Mn2+) has been introduced as a paramagnetic contrast agent for neuroaxonal tract tracing using magnetic resonance imaging (MRI). Extending previous work on murine brain in situ, this study demonstrates mapping of the visual pathway in living rats (n=6) at 2.35 T (Bruker, DBX). Multiple follow-up examinations at high spatial resolution (195 x 195 x 125μm3) were performed with use of T1-weighted 3D gradient-echo MRI (TR/TE=15/4.2ms, flip angle 25°) at 8h, 24h, 48h, and 72h after intraocular Mn2+ injection (0.1μl of 1M aqueous MnCl2). Best results were obtained at 24h post-injection revealing a continuous pattern of anterograde labeling from the retina, optic nerve, and chiasm to the contralateral optic tract, the dorsal and ventral lateral geniculate nucleus, the superior colliculus and its brachium, the olivary pretectal nucleus, the nucleus of the optic tract, and the suprachiasmatic nucleus. The approach is expected to advance studies of neuroaxonal function in behaving animals with applications in developmental neurobiology.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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