Neuroscience 2000 Abstract
| Presentation Number: | 796.7 |
|---|---|
| Abstract Title: | Cellular-resolution magnetic resonance imaging of an awake, behaving animal. |
| Authors: |
Jasanoff, A.*1
; Meister, M.2
; Cory, D. G.3
1Whitehead Institute, Cambridge, MA 2Molecular & Cellular Biology, Harvard University, Cambridge, MA 3Nuclear Engineering, Massachusetts Institute of Technology, Cambridge, MA |
| Primary Theme and Topics |
F. Sensory Systems - 77. Retina and photoreceptors |
| Secondary Theme and Topics | I. Neural Basis of Behavior<br />- 107. Learning and memory: systems and functions-animals |
| Session: |
796. Sensory systems III Poster |
| Presentation Time: | Thursday, November 9, 2000 10:00 AM-11:00 AM |
| Location: | Hall G-J |
| Keywords: | MRI, microscopy, blowfly, anatomy |
We present the first cellular-resolution magnetic resonance (MRI) images of an alert animal. Unanesthetized blowflies were inserted into an efficient home-built gradient probe in a 14.1 T magnet, and images of (20-40 μm)3 voxel size—smaller than the dimensions of many cells in the fly’s brain—were obtained in several hours. The images have exceptional contrast-to-noise levels, and comparison with histology-based anatomical information shows that the MRI microscopy faithfully represents detailed patterns of musculature and nervous tissue common to muscoid species. Even at the highest resolutions we explored, morphological detail was pronounced in the apparent absence of instabilities or movement-related artifacts frequently observed during in vivo imaging of animal specimens. Cellular-level structural information is present in the images and rigorously demonstrable by cross-correlation of the data in Fourier space. Our work constitutes a methodological prerequisite for functional imaging studies at cellular resolution.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2000 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2000. Online.
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