Neuroscience 2002 Abstract
| Presentation Number: | 817.7 |
|---|---|
| Abstract Title: | Clonal Analysis of Adult Bone Marrow Stromal Cells and Behavior after Transplantation into the Adult Rat Brain. |
| Authors: |
Coyne, T. M.*1
; Woodbury, D.1
; Reynolds, K.1
; Black, I. B.1
1Dept Neurosci & Cell Biol, Univ Med & Dentistry, New Jersey, Piscataway, NJ |
| Primary Theme and Topics |
Development - Neurogenesis and Gliogenesis -- Cell lineage and cell fate specification |
| Session: |
817. Neurogenesis and gliogenesis: cell lineage and cell fate IV Poster |
| Presentation Time: | Thursday, November 7, 2002 10:00 AM-11:00 AM |
| Location: | Hall A2-B3 A-1 |
| Keywords: | differentiation, Transplantation, MSC |
Until recently adult somatic stem cells were thought to differentiate only into cells of their resident tissues. Several reports have suggested that adult stem cells are more plastic than previously appreciated, even differentiating into cell types developmentally derived from different germ layers. We reported that Marrow Stromal Cells (MSCs), which normally differentiate into mesenchymal derivatives (bone, cartilage, muscle, tendon and fat) are able to differentiate into neurons, classical ectodermal cells. To begin characterizing mechanisms regulating transgerminal differentiation of MSCs, we previously examined gene expression in undifferentiated populations in dissociated culture. Surprisingly, the CD11b-/CD34-/CD45-/CD44+/CD77+/CD91+ population expressed genes representing all primary embryonic germ layers (SFN Abstract, 2001) Since the MSCs most probably comprise a heterogeneous population, we performed clonal analysis in the present studies. A clonal population, derived from a single cell, express genes representative of all three germ layers, including ceruloplasmin (En), SM22 (M), and syntaxin (Ex). Our observations indicate individual MSC clones express genes characteristic of endodermal, ectodermal as well as mesodermal genes and are therefore already multidifferentiated. We have recently extended our in vivo analysis, assessing behavior of MSCs in the adult rat brain.
Supported by NIH (HD 23315).
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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