Neuroscience 2005 Abstract
| Presentation Number: | 724.2 |
|---|---|
| Abstract Title: | Endogenous and exogenous cannabinoids potentiate glycine receptor-mediated responses through a CB receptor independent mechanism in <i>Xenopus</i> oocytes. |
| Authors: |
Sun, H.*1
; Hejazi, N.1
; Oz, M.2
; Zhang, L.1
1NIAAA, NIH, Rockville, MD 2MD, 5625 Fisher Lane, 20852, |
| Primary Theme and Topics |
Neural Excitability, Synapses, and Glia: Cellular Mechanisms - Ligand-Gated Ion Channels -- Glycine receptors |
| Session: |
724. Glycine Receptors Poster |
| Presentation Time: | Tuesday, November 15, 2005 2:00 PM-3:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # E72 |
| Keywords: | GLYCINE, RECEPTOR, CANNABINOIDS, POTENTIATION |
Anandamide and delta 9-tetrahydrocannabinol (THC) are endogenous and exogenous ligands for cannabinoid (CB) receptors. While most of anandamide and THC actions are thought to be mediated through endogenous cannabinoid type1 (CB1) receptors, there is evidence that some of cannabinoid-induced behaviors are independent of CB1 receptors. Here, we report that both anandamide and THC increased the amplitude of current activated by glycine in Xenopus oocytes expressing human homomeric and heromeric glycine receptors in a concentration-dependent manner over a concentration range from 30 to 3000 nM. The EC50 values of the THC and ANA concentration-response curves were 86 ± 9 nM and 319 ± 10 nM for the α1 subunits; and 73 ± 8 nM and 318 ± 14 nM for the α1β1 subunits. ANA and THC did not alter GABAA receptor-mediated responses, suggesting that the potentiation by ANA and THC is specific for the glycine receptors. The maximum potentiation by ANA and THC was observed at the lowest concentration of glycine. With increasing glycine concentrations, the magnitude of potentiation decreased. Preapplication of 1 µM SR141,716A, a selective CB1 receptor antagonist, did not significantly affect the ANA and THC-induced potentiating effects on glycine receptor-mediated responses. These observations have suggested that ANA and THC at physiological and pharmacological concentrations can enhance human glycine receptor function through a CB receptor-independent mechanism.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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