Neuroscience 2002 Abstract
| Presentation Number: | 796.7 |
|---|---|
| Abstract Title: | Using functional MRI and concurrent EEG to track the initiation and early progression of seizure in a conscious rat. |
| Authors: |
Brevard, M. E.*1
; Ives, J. R.2
; Duong, T. Q.1
; King, J. A.1
; Ferris, C. F.1
1Center for Comparative Neuroimaging, University of Massachusetts Medical School, Worcester, MA 2Neurology, Harvard Medical School, Boston, MA |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Epilepsy -- Human studies and animal models |
| Secondary Theme and Topics | Techniques in Neuroscience<br />- Staining, tracing and imaging techniques |
| Session: |
796. Epilepsy: networks and circuitry Poster |
| Presentation Time: | Wednesday, November 6, 2002 3:00 PM-4:00 PM |
| Location: | Hall A2-B3 W-16 |
| Keywords: | FMRI, SEIZURE, EPILEPSY, ANIMAL MODEL |
This study utilized functional MRI to evaluate the early stages of a kainic acid induced seizure in a fully conscious rat. Seizure activity was confirmed by EEG, and blood oxygen level dependant (BOLD) signal was temporally consistent with this activity.
Male SD rats where anesthetized using medetomidine (Pfizer) and ketamine and EEG electrodes were implanted. The animal was then placed into a special animal restraint/Rf coil system (Insight Neuroimaging systems). This system was put into a 4.7T MRI (Bruker) and the animal was awoken with atipamizole (Pfizer). Physiologic parameters were recorded for the entire duration of the experiment.
Anatomical images were acquired before and after the seizure. A 45 repetition functional sequence was acquired with 14 baseline repetitions (3 minutes) followed by an IP injection of kainic acid (10mg/kg). Seizure activity was noted via EEG within a minute. The anatomical images were subtracted to confirm that the awake animal remained immobile during the experiment. All MRI sequences were Fast Spin Echo.
The BOLD signal histogram in figure 1B, of the green cortical region in A, shows increases in activation of the cortex during EEG recorded seizures at points 16-20, 25-26, 31-33, and 40-41.
Male SD rats where anesthetized using medetomidine (Pfizer) and ketamine and EEG electrodes were implanted. The animal was then placed into a special animal restraint/Rf coil system (Insight Neuroimaging systems). This system was put into a 4.7T MRI (Bruker) and the animal was awoken with atipamizole (Pfizer). Physiologic parameters were recorded for the entire duration of the experiment.
Anatomical images were acquired before and after the seizure. A 45 repetition functional sequence was acquired with 14 baseline repetitions (3 minutes) followed by an IP injection of kainic acid (10mg/kg). Seizure activity was noted via EEG within a minute. The anatomical images were subtracted to confirm that the awake animal remained immobile during the experiment. All MRI sequences were Fast Spin Echo.
The BOLD signal histogram in figure 1B, of the green cortical region in A, shows increases in activation of the cortex during EEG recorded seizures at points 16-20, 25-26, 31-33, and 40-41.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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