Neuroscience 2004 Abstract
| Presentation Number: | 694.20 |
|---|---|
| Abstract Title: | Stereological analysis of age-related changes in hippocampal and brain volumes from MRI and processed tissue in APP/PS1 mice. |
| Authors: |
Manaye, K. F.*1
; Wang, P.2
; O'Neil, J.1
; Oei, A.2
; Song, H.2
; Ingram, D. K.4
; Mouton, P. R.3
1Dept Physiol & Biophysics, Howard Univ Col Med, Washington, DC 2DC, 520 W St NW, 20059, 3USA, 520 W St NW, 20059, 4Radiology, 520 W St NW, 20059, |
| Primary Theme and Topics |
Techniques in Neuroscience - Data analysis, physiological methods, statistics |
| Secondary Theme and Topics | Techniques in Neuroscience<br />- Staining, tracing and imaging techniques |
| Session: |
694. Techniques: Brain Imaging Methods II Poster |
| Presentation Time: | Tuesday, October 26, 2004 11:00 AM-12:00 PM |
| Location: | San Diego Convention Center - Hall A-H, Board # FFF32 |
| Keywords: | stereology, MRI, Hippocampus, APP/PS1mice |
Stereological estimates of human cortex and whole brain volumes at autopsy reveal only slight age-related changes. Autopsied brains from patients suffering neurological disease, including acquired immunodeficiency syndrome (AIDS) and Alzheimer's disease (AD), show significant loss (atrophy) of cortical and whole brain volumes compared to age-matched controls, with strong correlation to dementia scores in AD (Mouton et al., 1998). Early detection of brain atrophy would improve understanding of the time course of neurodegeneration and accelerate the therapeutic management of these diseases. We used high-resolution magnetic resonance imaging (MRI) to visualize hippocampal and whole brain volumes of mice double transgenic for two mutant proteins associated with AD (amyloid precursor protein and presenilin-1). Young and aged dtg APP/PS1 and wild-type (WT) mice were imaged by spin-echo T1-weighted MRI techniques. Total volumes of hemispheres (Vbrain) and hippocampal formation (VHF) were estimated from systematic-random series of coronal MRI images using the point counting-Cavalieri method. The same method was used to estimate Vbrain and VHF on age-matched mice after perfusion and tissue processing. The findings for dtg APP/PS1 mice indicate reductions in VHF and no change in Vbrain compared to volume estimates in WT mice. Comparison of volumes from MRI images and fully processed tissue revealed consistent shrinkage due to agonal and tissue processing effects. Ongoing studies will determine whether the age-related changes in cortical brain volumes in dtg APP/PS1 mice mimic the patterns that occur in AD.
Supported by intramural and extramural funding from the U.S. Public Health Service. DAMD-17-00-1-0291 and RCMI/NCRR/NIH 2G12RRoo3048
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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